A novel acridine-linked phenanthroline ligand, N-(1,10-phenanthrolin-5-yl)acridine-9-carboxamide, (PAC) was synthesized by N,N’-dicyclohexylcarbodiimide (DCC) coupling between acridine-9-carboxylic acid and 1,10-phenanthroline-5-amine. A set of new complexes of general formulae [M(PAC)(N₃)₂] and [M(PAC)₂(N₃)₂] where M = Cu(II), Ni(II), and Co(II) metal ions were synthesized and characterised by spectroscopic, electrochemical, and thermogravimetric methods. Interactions of complexes with Calf Thymus DNA were investigated using UV–visible absorption studies, fluorescent intercalator displacement assays, viscosity measurements, and cyclic voltammetric studies in phosphate buffer at pH 7.2. All the synthesized complexes were found to be potential DNA-binding agents, with significant quenching of fluorescence with the addition of DNA. Hypochromic shifts of the absorption bands with well-defined isosbestic points were observed for all complexes in the presence of the nucleic acid and the binding constants (K_b) calculated revealed high binding affinities of the complexes. Viscosity and cyclic voltammetric measurements supported appreciable intercalative interactions between the complexes and DNA.

中文翻译：

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N-（1,10-菲咯啉-5-基）ac啶-9-羧酰胺的新型Cu（II），Ni（II）和Co（II）单配体和双配体配合物的合成，表征和DNA相互作用研究

通过N，N'-二环己基碳二亚胺（DCC）在a啶-9-羧酸之间的偶联反应合成了一种新型的idine啶连接的菲咯啉配体N-（1,10-菲林-5-基）ac啶-9-羧酰胺（PAC）和1,10-菲咯啉-5-胺 一组通式为[M（PAC）（N ₃）₂ ]和[M（PAC）₂（N ₃）₂ ]的新配合物，其中M 合成了Cu（II），Ni（II）和Co（II）金属离子，并通过光谱，电化学和热重分析法对其进行了表征。使用紫外线可见吸收研究，荧光嵌入剂置换分析，粘度测量和pH 7.2磷酸盐缓冲液中的循环伏安法研究了配合物与小牛胸腺DNA的相互作用。发现所有合成的复合物都是潜在的DNA结合剂，添加DNA后荧光显着猝灭。吸收带与观察到在核酸的存在的所有络合物和结合常数定义良好的等吸光点的低色素位移（ķ _b）的计算揭示了复合物的高结合亲和力。粘度和循环伏安法测量支持复合物与DNA之间明显的插入相互作用。