Abstract

The current work investigated the green and low-cost preparation of silver nanoparticles (AgNPs) using the aqueous extract from Launaea taraxacifolia leaf and studied its antimicrobial effects. The leaf extract was analysed in a gas chromatogram–mass spectrometer to assess the phytochemicals present. UV–Vis spectrophotometer was used to monitor the formation of AgNPs, the morphological assessment was performed by a scanning electron microscope, energy dispersive X-ray analysis was used to determine the elemental composition, the particle size and shape were studied using transmission electron microscopy, and the vibrational modes of bonds in the AgNPs were assessed by Fourier transformed infrared spectroscopy. The AgNPs produced were spherical and in a size range of 9–15.5 nm, monodispersed with a large surface area. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the AgNPs against Pseudomonas aeruginosa and Proteus mirabilis were very low. Against P. aeruginosa, the MIC was 0.10 mg/mL and the MBC was 0.15 mg/mL, while the MIC and MBC against P. mirabilis were 0.05 and 0.25 mg/mL, respectively. Therefore, the AgNPs prepared using L. taraxacifolia leaf extract showed high antibacterial activities and could be a candidate antimicrobial agent for biomedical applications.

Graphic Abstract

中文翻译：

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用太子参叶提取物制备单分散的具有细胞毒性的银纳米颗粒

摘要

当前的工作研究了使用来自Launaea taraxacifolia的水提物的绿色低成本纳米银的制备方法并研究了其抗菌作用。叶提取物在气相色谱-质谱仪中进行分析，以评估存在的植物化学物质。用UV-Vis分光光度计监测AgNP的形成，通过扫描电子显微镜进行形态学评估，使用能量色散X射线分析确定元素组成，使用透射电子显微镜研究颗粒大小和形状，用傅立叶变换红外光谱法评估了AgNPs中键的振动模式。产生的AgNP为球形，大小范围为9–15.5 nm，单分散，表面积大。AgNPs对铜绿假单胞菌和铜绿假单胞菌的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）奇异变形杆菌很低。对于铜绿假单胞菌，MIC为0.10 mg / mL，MBC为0.15 mg / mL，而针对奇异假单胞菌的MIC和MBC分别为0.05和0.25 mg / mL。因此，用蒲公英乳杆菌叶提取物制备的AgNPs具有很高的抗菌活性，可以作为生物医学应用的候选抗菌剂。

图形摘要