Fructans are polymers of fructose that are present as storage carbohydrates in various plants. Jerusalem artichoke (Helianthus tuberosus L.) contains a high amount of inulin. Two enzymes are involved in inulin biosynthesis. The sucrose:sucrose 1-fructosyltransferase (1-SST) enzyme mainly catalyzes the synthesis of 1-kestose from sucrose. In the next step, fructan:fructan 1-fructosyltransferase (1-FFT) catalyzes the synthesis of inulin from 1-kestose. In this study, the Ht1-SST and Ht1-FFT genes were isolated from Jerusalem artichoke and expressed in potato (Solanum tuberosum L.), either separately or together, via Agrobacterium-mediated transformation. Transgenic potato tubers overexpressing Ht1-SST accumulated 1-kestose to a high level (up to 3.36 mg/g), while tubers overexpressing both Ht1-SST and Ht1-FFT accumulated up to 3.14 mg/g short-chain inulin-type fructans, with the degree of polymerization (DP) ranging from 3 to 5, excluding high DP inulins. Transgenic potato plants accumulated fructo-oligosaccharides to a high level, following the fructan biosynthetic pathway of Jerusalem artichoke, and therefore present a high potential for the mass production of inulin through established potato breeding and cultivation methods.

中文翻译：

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菊芋（表达菊芋L.）fructosyltransferases和高果聚糖积累在马铃薯块茎

果聚糖是果糖的聚合物，其在各种植物中作为储存碳水化合物存在。菊芋（Helianthus tuberosus L.）含有大量菊粉。菊粉的生物合成涉及两种酶。蔗糖：蔗糖的1-果糖基转移酶（1-SST）酶主要催化由蔗糖合成的1-蔗糖。在下一步中，果聚糖：果聚糖1-果糖基转移酶（1-FFT）催化从1-蔗糖合成菊粉。在这项研究中，Ht1-SST和Ht1-FFT基因是从菊芋中分离出来的，并通过农杆菌介导的转化分别或一起在马铃薯（马铃薯）中表达。过度表达Ht1-SST的转基因马铃薯块茎累积了1个钾的高水平（最高3.36 mg / g），而过度表达Ht1-SST和Ht1-FFT的块茎累积了3个。14 mg / g短链菊粉型果聚糖，聚合度（DP）为3-5，高DP菊粉除外。继菊芋的果聚糖生物合成途径后，转基因马铃薯植物中的果糖寡糖积累量很高，因此，通过成熟的马铃薯育种和栽培方法，菊粉具有大规模生产菊粉的潜力。