当前位置: X-MOL 学术J. Plant Biochem. Biot. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Genome-wide identification and characterization of the aquaporin gene family in Medicago truncatula
Journal of Plant Biochemistry and Biotechnology ( IF 1.9 ) Pub Date : 2019-01-01 , DOI: 10.1007/s13562-018-0484-4
Xueyang Min , Honglin Wu , Zhengshe Zhang , Xingyi Wei , Xiaoyu Jin , Boniface Ndayambaza , Yanrong Wang , Wenxian Liu

Aquaporins (AQPs), or major intrinsic proteins (MIPs), constitute a large and diverse family of protein channel transporter in plants. However, the information about AQPs family is still poorly understood in the model legume species Medicago truncatula. Here, 46 AQPs were identified and characterized. Based on the phylogenetic analysis, these genes were assigned to five subfamilies, specifically as 10 plasma membrane intrinsic proteins, 12 tonoplast intrinsic proteins, 18 NOD26-like intrinsic proteins, 4 small basic intrinsic proteins and 2 uncharacterized intrinsic proteins. The essential information of AQPs, such as subcellular localization, gene structure, chromosomal localization, evolutionary stress and functional residues were systematically analyzed. In addition, in silico analysis of the gene expression profiles showed certain of tissue specificity and responsiveness to drought/salt stresses in some AQPs genes. The qRT-PCR results showed that MtPIP1;5 and MtTIP1;3 were significantly up-regulated under both drought and salinity stress conditions, indicated their important roles in response to abiotic stresses. The results could provide valuable information for the further functional analysis of the AQPs in M. truncatula and related legume species.

中文翻译:

全基因组苜蓿水通道蛋白基因家族的全基因组鉴定和表征

水通道蛋白(AQP)或主要内在蛋白(MIP)构成了植物中多种多样的蛋白通道转运蛋白家族。但是,在模型豆科植物紫花苜蓿中AQPs家族的信息仍然知之甚少。在这里,确定并表征了46个AQP。根据系统发育分析,将这些基因分配给五个亚家族,分别是10个质膜内在蛋白,12个液泡膜内在蛋白,18个NOD26样内在蛋白,4个小的基本内在蛋白和2个未表征的内在蛋白。AQP的基本信息对亚细胞定位,基因结构,染色体定位,进化应激和功能残基等进行了系统分析。另外,基因表达谱的计算机分析显示某些AQPs基因具有一定的组织特异性和对干旱/盐胁迫的响应能力。qRT-PCR结果表明,在干旱和盐胁迫条件下,MtPIP1; 5MtTIP1; 3均显着上调,表明它们在应对非生物胁迫中的重要作用。这些结果可为进一步分析t和相关豆科植物的AQPs提供有价值的信息。
更新日期:2019-01-01
down
wechat
bug