The recent isolation of an atoxigenic strain of Aspergillus flavus (Link) (Eurotiales: Trichocomaceae), which was virulent to all stages of Xylosandrus compactus (Eichhoff) (Coleoptera: Curculionidae) has generated interest to investigate virulence of the entomopathogenic fungus against other crop pests, and how it compares with established commercial biopesticides. In this study, we tested the pathogenicity and virulence of the atoxigenic A. flavus strain to Ootheca mutabilis (Sahlberg) (Coleoptera: Chrysomelidae) in the laboratory compared to the commercial Metarhizium anisopliae (Metschnikoff Sorokin) (Hypocreales: Clavicipitaceae; OD®, ICIPE 69). Serial dilutions of both entomopathogenic fungi (1 × 106–1 × 109 conidia/ml) were evaluated against adult O. mutabilis. Distilled water and cypermethrin 5% EC were included as controls. The treatments were replicated four times and the bioassay was repeated once. Both fungi at 1 × 109 conidia/ml killed 100% of tested O. mutabilis within seven days in the 1st and 2nd trials. The concentration of both fungi that killed 50% of O. mutabilis was the same in both trials (5.3 × 106 conidia/ml). The time taken by A. flavus and ICIPE 69 to kill 50% of O. mutabilis was not significantly different in the 1st trial (3.4 ± 0.1 days and 2.9 ± 0.4 days, respectively). However, ICIPE 69 took a significantly shorter time (2.1 ± 0.4 days) than A. flavus (3.6 ± 0.2 days) to kill 50% of O. mutabilis in the 2nd trial. We concluded that A. flavus is comparably as pathogenic and virulent (in terms of cumulative mortality and LC50) against O. mutabilis as ICIPE 69, and therefore both fungi may be viable biopesticides for commercialization against this pest.

中文翻译：

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黄曲霉（Eurotiales：Trichocomaceae）和商业ICIPE 69 Metarhizium anisopliae（Hypocreales：Clavicipitaceae）的无毒株对豆叶甲虫Ootheca mutabilis（鞘翅目：Chrysomelidae）的毒力比较

最近分离出一种对黄曲霉（Eichhoff）（鞘翅目：仙茅科）的所有阶段都具有毒性的黄曲霉（Link）（Eurotiales：Trichocomaceae）的无毒菌株引起了人们对研究昆虫病原真菌对其他作物害虫的毒力的兴趣，以及它如何与成熟的商业生物农药进行比较。在这项研究中，我们在实验室中测试了无毒黄曲霉菌株对 Ootheca mutabilis (Sahlberg)（鞘翅目：Chrysomelidae）的致病性和毒力与商业绿僵菌（Metschnikoff Sorokin）（Hypocreales：Clavicipitaceae；CIPE OD® 69）。对两种昆虫病原真菌（1 × 106–1 × 109 分生孢子/ml）的系列稀释液针对成年 O. mutabilis 进行了评估。包括蒸馏水和氯氰菊酯 5% EC 作为对照。处理重复四次，生物测定重复一次。在第 1 次和第 2 次试验中，1 × 109 分生孢子/毫升的两种真菌在 7 天内杀死了 100% 的被测 O. mutabilis。在这两个试验中，杀死 50% 的 O. mutabilis 的两种真菌的浓度相同（5.3 × 106 分生孢子/ml）。A. flavus 和 ICIPE 69 杀死 50% 的 O. mutabilis 所需的时间在第一次试验中没有显着差异（分别为 3.4 ± 0.1 天和 2.9 ± 0.4 天）。然而，在第二次试验中，ICIPE 69 比黄曲霉（3.6 ± 0.2 天）花费了明显更短的时间（2.1 ± 0.4 天）来杀死 50% 的变异链球菌。我们得出结论，A. flavus 对 O. mutabilis 的致病性和毒性（在累积死亡率和 LC50 方面）与 ICIPE 69 相当，