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Differences in dissolved phosphate in shallow-lake waters as determined by spectrophotometry and ion chromatography
Limnology ( IF 1.6 ) Pub Date : 2019-04-06 , DOI: 10.1007/s10201-019-00574-2
Rong Yi , Peixue Song , Xin Liu , Masahiro Maruo , Syuhei Ban

Phosphorus (P) plays important roles in aquatic ecosystems, but accurately determining phosphorus species (particularly bioavailable phosphate) is difficult. When phosphate concentrations are spectrophotometrically measured as soluble reactive P (SRP), the actual values are often overestimated. Ion chromatography is one method for accurately measuring orthophosphate concentrations. To clarify how the spectrophotometrical measurement overestimates actual phosphate concentrations, we compared estimates of phosphate concentrations in lake waters using both methods. SRP and orthophosphate concentrations in water samples collected from four shallow lakes around Lake Biwa, Japan, were determined monthly for 1 year by spectrophotometry and ion chromatography, respectively. SRP concentrations were frequently higher than those for orthophosphate in all lakes throughout the study period, suggesting that SRP and orthophosphate are not equivalent, although a significant relationship was found between them. Orthophosphate/SRP (ortho/SRP) ratios varied spatiotemporally in all lakes (range 0.11–1.04; mean 0.56), being well predicted by biological-mediated relevant parameters using a stepwise multiple logistic regression analysis (R2 = 0.76). The results implied that organic matter associated with biological activity contributes to the variability in ortho/SRP ratios. Such large variability in ortho/SRP ratios emphasizes the importance of accurate orthophosphate estimates for understanding P dynamics in aquatic ecosystems.

中文翻译:

分光光度法和离子色谱法测定浅湖水中溶解磷酸盐的差异

磷(P)在水生生态系统中起着重要的作用,但是要准确确定磷的种类(尤其是可生物利用的磷酸盐)却很困难。当用分光光度法测量磷酸盐浓度作为可溶性反应性P(SRP)时,实际值通常被高估了。离子色谱法是一种准确测量正磷酸盐浓度的方法。为了阐明分光光度法测量是如何高估实际磷酸盐浓度的,我们使用两种方法比较了湖水中磷酸盐浓度的估算值。通过分光光度法和离子色谱法分别测定了从日本琵琶湖附近四个浅湖收集的水样中SRP和正磷酸盐的浓度,为期1年,每月一次。在整个研究期间,所有湖泊中SRP的浓度通常都高于正磷酸盐的浓度,这表明SRP和正磷酸盐并不等效,尽管两者之间存在显着的关系。在所有湖泊中,正磷酸盐/ SRP(正/ SRP)之比随时间呈时空变化(范围为0.11-1.04;平均值为0.56),并通过生物介导的相关参数使用逐步多元logistic回归分析很好地预测(R 2  = 0.76)。结果表明,与生物活性有关的有机物有助于邻/ SRP比的变化。正/ SRP比值的如此大的变化强调了准确的正磷酸盐估算值对于理解水生生态系统中磷动力学的重要性。
更新日期:2019-04-06
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