The objective of this investigation was to determine the in vitro effect of two common digestive enzymes, amylase and pepsin, and pH on the integrity of the RI dsRNA of bell pepper endornavirus (BPEV) and Cucumis melo endornavirus (CmEV) evaluated by gel electrophoresis and reverse-transcription PCR (RT-PCR). We conducted experiments on the in vitro effect of two common digestive enzymes, amylase and pepsin, and pH on the structural integrity of the replicative intermediate (RI) dsRNA of bell pepper endornavirus (BPEV) and Cucumis melo endornavirus (CmEV), evaluated by gel electrophoresis and reverse-transcription polymerase chain reaction. The effect of the amylase, pepsin, and pH treatments on the dsRNA of both viruses was similar. Amylase did not appear to affect the structural integrity of the dsRNA. In contrast, gel electrophoresis analysis of pepsin-treated dsRNA samples showed an abnormal electrophoretic migration and evidence of partial dsRNA degradation. DsRNAs from both fruits were partially degraded when exposed to a pH value of 2.0 and completely degraded at a pH value of 1.0. The results of this investigation suggest that when exposed to pepsin and pH values lower than 2.0, the RI of BPEV and CmEV lose their structural integrity. Therefore, when consuming endornavirus-infected bell pepper or melon, our digestive organs are exposed to both fragmented and full RI dsRNA of these two viruses.

中文翻译：

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体外条件下两种消化酶和pH值对甜椒和甜瓜内膜病毒dsRNA的影响

这项研究的目的是确定两种常见的消化酶（淀粉酶和胃蛋白酶）和pH值对通过凝胶电泳和磷酸化评估的灯笼椒内膜病毒（BPEV）和瓜型内膜内膜病毒（CmEV）RI dsRNA完整性的体外影响。逆转录PCR（RT-PCR）。我们通过凝胶评估了两种常见的消化酶（淀粉酶和胃蛋白酶）以及pH对甜椒内膜病毒（BPEV）和黄瓜甜瓜内膜病毒（CmEV）的复制中间体（RI）dsRNA结构完整性的体外影响的实验。电泳和逆转录聚合酶链反应。淀粉酶，胃蛋白酶和pH处理对两种病毒的dsRNA的影响相似。淀粉酶似乎不影响dsRNA的结构完整性。相反，胃蛋白酶处理的dsRNA样品的凝胶电泳分析显示异常的电泳迁移和部分dsRNA降解的证据。当暴露于2.0的pH值时，两个果实的DsRNA均部分降解，而在pH值为1.0时则完全降解。这项研究的结果表明，当暴露于胃蛋白酶且pH值低于2.0时，BPEV和CmEV的RI会失去其结构完整性。因此，当食用感染了核糖核酸的甜椒或甜瓜时，我们的消化器官会同时暴露于这两种病毒的片段RI和完整RI dsRNA。这项研究的结果表明，当暴露于胃蛋白酶且pH值低于2.0时，BPEV和CmEV的RI会失去其结构完整性。因此，当食用感染了核糖核酸的甜椒或甜瓜时，我们的消化器官会同时暴露于这两种病毒的片段RI和完整RI dsRNA。这项研究的结果表明，当暴露于胃蛋白酶且pH值低于2.0时，BPEV和CmEV的RI会失去其结构完整性。因此，当食用感染了核糖核酸的甜椒或甜瓜时，我们的消化器官会同时暴露于这两种病毒的片段RI和完整RI dsRNA。