In this study, the antimelanogenic activity of piperlongumine in murine B16F10 melanoma cells and zebrafish was investigated, and its mode of antimelanogenic action was elucidated using quantitative reverse transcription-polymerase chain reaction. A melanocyte-stimulating hormone (α-MSH, 200 nM) was used to induce melanin production in B16F10 melanoma cells, and kojic acid (200 μM) was used as a positive control. Piperlongumine had no inhibitory effects on cell growth at the treated concentrations (3 and 6 μM), and it significantly reduced total melanin production. Piperlongumine decreased the expression of Mitf, Tyr, Trp-1, and Trp-2 and tyrosinase activity was also dramatically reduced by the piper amide addition under α-MSH treatment. With these findings, zebrafish embryos were used to confirm antimelanogenic activity of piperlongumine, and it showed the potent antimelanogenic activity at the concentration of 1 μM. Altogether, piperlongumine has potent antimelanogenic activity, and these results support it as a candidate for natural depigmentation agent in a cosmetic and pharmaceutical industries.

中文翻译：

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衍生自piperlongumine的Antimelanogenic活动荜茇在体外鼠B16F10黑素瘤细胞和体内斑马鱼胚胎：脱色作用的分子模式

在这项研究中，研究了哌隆丁胺在鼠B16F10黑色素瘤细胞和斑马鱼中的促炭疽活性，并通过定量逆转录-聚合酶链反应阐明了其促炭疽作用的模式。使用刺激黑色素细胞的激素（α-MSH，200 nM）诱导B16F10黑色素瘤细胞中黑色素的产生，并使用曲酸（200μM）作为阳性对照。在处理浓度（3和6μM）下，哌隆丁胺对细胞生长没有抑制作用，并且显着降低了总黑色素的产生。在α-MSH处理下，添加哌啶酰胺可显着降低Piperlongumine的Mitf，Tyr，Trp-1和Trp-2的表达，并大大降低酪氨酸酶的活性。有了这些发现，斑马鱼的胚胎被用于确认哌隆明碱的促炭疽活性，并且在1μM的浓度下显示出了强大的促炭疽活性。总而言之，哌隆丁胺具有有效的促炭疽活性，这些结果支持其在化妆品和制药行业中作为天然色素脱色剂的候选者。