Abstract

Magnetic molecularly imprinted polymers (MMIPs) were synthesized on Fe₃O₄ using methacrylic acid (MAA) and acrylamide (AA) as monomers, ethylene glycol dimethacrylate (EGDMA) as cross-linker and rutin as a template under nitrogen atmosphere in about 90 min. Fourier transform infrared spectroscopy, electron microscopy, vibrating sample magnetometer (VSM), thermo-gravimetric analysis (TGA), and Brunauer–Emmett–Teller (BET) surface area analysis were used for particles characterization. Data showed that MMIPs was well synthesized and had high porosity. Synthetic MMIPs using methacrylic acid had much better performance than synthetic MMIPs using acrylamide. MMIPs were used for rapid separation and determination of rutin. MMIPs solid-phase extraction coupled with UV–visible spectrophotometer method was established for rutin analysis in green tea sample. The developed method had good linear dynamic range (1–90 µM, R² = 0.996), with a detection limit as low as 0.961 µM, precision (1.5–2.1%) and accuracy (103.1–110.9%).

Graphic abstract

Separation of rutin by magnetic molecular-imprinted polymers

中文翻译：

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磁性分子印迹聚合物在绿茶中芦丁测定中的应用

摘要

在Fe ₃ O ₄上合成了磁性分子印迹聚合物（MMIP）以甲基丙烯酸（MAA）和丙烯酰胺（AA）为单体，以乙二醇二甲基丙烯酸酯（EGDMA）为交联剂，以芦丁为模板，在氮气气氛下约90分钟。傅立叶变换红外光谱，电子显微镜，振动样品磁力计（VSM），热重分析（TGA）和Brunauer-Emmett-Teller（BET）表面积分析用于颗粒表征。数据表明，MMIPs合成良好且具有高孔隙率。与使用丙烯酰胺的合成MMIP相比，使用甲基丙烯酸的合成MMIP具有更好的性能。MMIP用于快速分离和测定芦丁。建立了MMIPs固相萃取结合紫外可见分光光度计方法对绿茶样品中的芦丁进行分析。所开发的方法具有良好的线性动态范围（1–90 µM，R ²  = 0.996），检出限低至0.961 µM，精度（1.5–2.1％）和精度（103.1–110.9％）。

图形摘要

磁性分子印迹聚合物分离芦丁