Abstract

Rice is a major dietary source of essential mineral nutrients and toxic elements (aka ionome) for humans. However, the genetic basis underlying the variation in ionome is still largely unknown. Here, we mapped 51 and 61 quantitative trait loci (QTLs) controlling the concentrations of 13 and 15 elements in rice (Oryza sativa L.) grain and straw, respectively, using a recombinant inbred lines (RILs) that were grown at three different field sites in 3 years. Several QTLs were repeatedly detected in both grain and straw or in multiple years; the resulting 87 unique QTLs with 17 of them (20%) were co-localized with previously reported corresponding QTLs and 70 were novel ionomic QTLs. At least, 14 genomic clusters that controlled the concentrations of multiple elements were identified. Furthermore, we identified a molybdate transporter gene OsMOT1;1 as the putative causal gene for a QTL controlling molybdenum concentration in both straw and grain. QTL analyses based on the concentrations of multiple elements in both grain and straw of RIL population grown in three field sites in 3 years allow us to identify tissue common QTLs and reproducible QTLs that were validated in multiple years. The identification of ionomic QTLs will be useful in revealing the molecular mechanisms underlying the accumulation of elements in rice and providing the opportunity to reduce the accumulation of toxic elements and enrich the accumulation of beneficial elements in rice grain.

中文翻译：

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水稻籽粒和稻草中基因组数量性状基因座的遗传图谱显示，OsMOT1; 1是钼QTL qMo8的推定致病基因

摘要

稻米是人体必需的主要矿物质营养物质和有毒元素（又名离子群）的主要饮食来源。然而，离子组变异背后的遗传基础仍是未知之数。在这里，我们映射51和61的数量性状基因座（QTL）控制13的浓度和在水稻15个元素（稻L.）谷物和稻草，分别使用重组自交系（RIL），在3年中分别在三个不同的田间种植。在谷物和稻草中或多年中反复检测到几个QTL；得到的87个独特QTL与其中先前报道的相应QTL共定位，其中17个（占20％）与新的基因组QTL共定位。至少鉴定出14个控制多种元素浓度的基因组簇。此外，我们鉴定了钼酸盐转运蛋白基因OsMOT1; 1作为控制稻草和谷物中钼浓度的QTL的推定原因基因。基于3年中在三个田间种植的RIL种群的谷物和稻草中多种元素的浓度进行的QTL分析，使我们能够识别经过多年验证的组织常见QTL和可再现QTL。蛋白质组QTL的鉴定将有助于揭示水稻中元素积累的分子机制，并为减少有毒元素的积累和丰富水稻籽粒中有益元素的积累提供机会。