In this study we tested a newly-developed instrument (an automatized DNA extractor MAxWell16-Promega), in order to test if DNA isolation can be performed more efficiently on feacal samples collected from three different species: bears, wolfs and capercaillies. We also evaluate a simple method for samples preservation in the field, which include the preservation of faeces in vials filled with ethanol (90%). This method we have used is fast and simple. The time spent for isolating DNA from each batch of 16 samples is approximately 38 min, no further processes needed. The extracted DNA can be used immediately in the downstream analyses. No further wash of the samples, or other purification method is needed. The DNA should not be diluted for the amplification step, likewise the PCR product is not recommended to be diluted for the fragment analysis step.

在这项研究中，我们测试了一种新开发的仪器（自动化的DNA提取器MAxWell16-Promega），以测试是否可以更有效地对从三种不同物种（熊，狼和Capercaillies）采集的粪便样品进行DNA分离。我们还评估了一种在现场保存样品的简单方法，其中包括将粪便保存在装有乙醇（90％）的小瓶中。我们使用的这种方法既快速又简单。从每批16个样品中分离DNA所花费的时间约为38分钟，无需进一步处理。提取的DNA可立即用于下游分析。无需进一步洗涤样品或其他纯化方法。DNA不应稀释用于扩增步骤，