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Shifts in microbial community composition in tannery-contaminated soil in response to increased gamma radiation
Annals of Microbiology ( IF 3 ) Pub Date : 2019-12-23 , DOI: 10.1007/s13213-019-01541-z
Mohammad A. A. Al-Najjar , Majed M. Albokari

Contaminated sites from man-made activities such as old-fashioned tanneries are inhabited by virulent microorganisms that exhibit more resistance against extreme and toxic environmental conditions. We investigated the effect of different Gamma radiation doses on microbial community composition in the sediment of an old-fashioned tannery. Seven samples collected from the contaminated sites received different gamma radiation doses (I = 0.0, II = 5, III = 10, VI = 15, V = 20, VI = 25, and VII = 30 kGy) as an acute exposure. The shift in microbial community structure was assessed using the high throughput 454 pyrosequencing. Variations in diversity, richness, and the shift in operational taxonomic units (OTUs) were investigated using statistical analysis. Our results showed that the control sample (I) had the highest diversity, richness, and OTUs when compared with the irradiated samples. Species of Halocella, Parasporobacterium, and Anaerosporobacter had the highest relative abundance at the highest radiation dose of 30 kGy. Members of the Firmicutes also increased by 20% at the highest radiation dose when compared with the control sample (0.0 kGy). Representatives of Synergistetes decreased by 25% while Bacteroidetes retained a steady distribution across the range of gamma radiation intensities. This study provides information about potential “radioresistant” and/or “radiotolerant” microbial species that are adapted to elevated level of chemical toxicity such as Cr and Sr in tannery. These species can be of a high biotechnological and environmental importance.

中文翻译:

制革厂污染的土壤中微生物群落组成的变化对伽马射线辐射的响应

人为活动(例如老式制革厂)造成的污染场所被有毒微生物居住,这些微生物对极端和有毒的环境条件表现出更大的抵抗力。我们调查了不同剂量的γ辐射对老式皮革厂沉积物中微生物群落组成的影响。从受污染的地点收集的七个样品接受了不同的伽马辐射剂量(I = 0.0,II = 5,III = 10,VI = 15,V = 20,VI = 25和VII = 30 kGy)作为急性暴露。使用高通量454焦磷酸测序评估了微生物群落结构的变化。使用统计分析调查了多样性,丰富度和操作生物分类单位(OTU)的变化。我们的结果表明,对照样品(I)具有最高的多样性,丰富度,与辐照样品比较时的OTU和OTU。在30 kGy的最高辐射剂量下,嗜盐杆菌,副孢杆菌和厌气杆菌的种类具有最高的相对丰度。当与对照样品(0.0 kGy)相比时,在最高辐射剂量下,Firmicutes的成员也增加了20%。协同菌的代表下降了25%,而拟杆菌的代表则在整个γ辐射强度范围内保持稳定的分布。这项研究提供了有关潜在的“辐射抗性”和/或“辐射抗性”微生物物种的信息,这些微生物适应了化学毒性水平的升高,例如制革厂中的Cr和Sr。这些物种可能具有很高的生物技术和环境重要性。在30 kGy的最高辐射剂量下,厌氧孢杆菌的相对丰度最高。当与对照样品(0.0 kGy)相比时,在最高辐射剂量下,Firmicutes的成员也增加了20%。协同菌的代表下降了25%,而拟杆菌的代表在整个γ辐射强度范围内保持稳定分布。这项研究提供了有关潜在的“辐射抗性”和/或“辐射抗性”微生物物种的信息,这些微生物物种适应了制革厂中较高的化学毒性水平,例如Cr和Sr。这些物种可能具有很高的生物技术和环境重要性。在30 kGy的最高辐射剂量下,厌氧孢杆菌的相对丰度最高。当与对照样品(0.0 kGy)相比时,在最高辐射剂量下,Firmicutes的成员也增加了20%。协同菌的代表下降了25%,而拟杆菌的代表在整个γ辐射强度范围内保持稳定分布。这项研究提供了有关潜在的“辐射抗性”和/或“辐射抗性”微生物物种的信息,这些微生物物种适应了制革厂中较高的化学毒性水平,例如Cr和Sr。这些物种可能具有很高的生物技术和环境重要性。协同菌的代表下降了25%,而拟杆菌的代表在整个γ辐射强度范围内保持稳定分布。这项研究提供了有关潜在的“辐射抗性”和/或“辐射抗性”微生物物种的信息,这些微生物物种适应了制革厂中较高的化学毒性水平,例如Cr和Sr。这些物种可能具有很高的生物技术和环境重要性。协同菌的代表下降了25%,而拟杆菌的代表在整个γ辐射强度范围内保持稳定分布。这项研究提供了有关潜在的“辐射抗性”和/或“辐射抗性”微生物物种的信息,这些微生物物种适应了制革厂中较高的化学毒性水平,例如Cr和Sr。这些物种可能具有很高的生物技术和环境重要性。
更新日期:2020-04-18
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