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The Developmental Stages of Sugarcane Stalk are Equivalent between Plants of Different Chronological Ages
Tropical Plant Biology ( IF 2 ) Pub Date : 2019-08-22 , DOI: 10.1007/s12042-019-09240-4
Donna Glassop , Mark P. Hodson , Panagiotis K. Chrysanthopoulos , Anne Rae

Sugarcane stalks sequentially produce internodes, with the most immature and mature internodes at the top and base of the stalk, respectively. Resulting in a range of internodes at different developmental stages in each stalk. When sampling similar developmental internodes, replicate plants may differ in chronological age due to various reasons, including different germination rates, regeneration from tissue culture, and treatments affecting growth. To date, there has been no research to test if internodes of the same developmental age are equivalent between plants of different chronological age, for example to demonstrate that internode 4 from a 6-month-old plant is functionally equivalent to internode 4 from a 9-month-old plant. Internodes 4, 6 and 11 were assessed in two varieties, Q117 and Q208, across a range of chronological ages from 6 to 9 months. This study utilised a combination of tissue morphology measurements, gene expression and metabolite abundance results to discern chronological/developmental variation. Some genes and metabolites were measured in both varieties and some were unique to each variety. When results from the two varieties were pooled together the internodes had more overlap between samples; highlighting that the uniqueness between the varieties was greater than internode similarity or that a different range of genes/metabolites is required when comparing different varieties. Analysis of the measurements for each variety independently did result in similar internode samples clustering together regardless of chronological age, with minimal overlap. These results illustrate that samples of similar developmental stage (± 1 internode) are similar between stalks of different chronological age. With this knowledge, researchers can now reliably compare internodes from plants of different chronological age, with confidence that results are not confounded by plant age.

中文翻译:

不同年代的植物之间甘蔗茎的发育阶段是等效的

甘蔗茎依次产生节间,最不成熟和成熟的节间分别在茎的顶部和底部。导致每根茎处于不同发育阶段的节间范围。当采样相似的发育节间时,由于各种原因,包括不同的发芽率,组织培养的再生以及影响生长的处理方法,复制植物的年代可能会有所不同。迄今为止,还没有研究测试相同发育年龄的节间在不同时间年龄的植物之间是否相等,例如,证明来自六个月大的植物的节间4在功能上等同于来自9个月大的植物的节间4。月大的植物。在两个品种Q117和Q208中评估了节点4、6和11 年龄从6到9个月不等。这项研究利用组织形态学测量,基因表达和代谢产物丰度结果的组合来识别时间/发育变化。在两个品种中都测量了一些基因和代谢产物,而每个品种都有一些独特。当将两个品种的结果汇总在一起时,节点间的样本之间会有更多的重叠;强调品种之间的唯一性大于节间相似性,或者在比较不同品种时需要不同范围的基因/代谢产物。对每个品种的测量值进行独立分析确实会导致类似的节点间样本聚集在一起,而与按时间顺序排列的年龄无关,且重叠最小。这些结果说明,在不同年代的茎之间,相似发育阶段(节间数为±1)的样本相似。有了这些知识,研究人员现在可以可靠地比较不同年代的植物的节间,并确信结果不会因植物的年龄而混淆。
更新日期:2019-08-22
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