Objective

The objective of this study was to compare the activity and biological function of leukocytes isolated using apheresis platelet leukoreduction system chambers (LRSC), whole blood leukoreduction filters (LRF), and leukocytes in unfiltered peripheral whole blood (WB).

Methods

Peripheral blood mononuclear cells (PBMCs) and granulocytes were obtained by density gradient centrifugation using recovery filters and WB. Flow cytometry was used to detect the activity, phenotype, and apoptosis ratio of each cell subtype.

Results

The proportion of lymphocytes obtained from PBMCs was similar when using the two different filters as compared to traditional isolation; however, there were significant differences between the monocytes and granulocytes. The phenotypic frequency of lymphocytes was similar, but the apoptosis rate of lymphocytes from the two filters was slightly higher. Additionally, monocytes isolated via the three sources were able to be induced into dendritic cells expressing specific molecules; Granulocytes isolated from the LRF showed a lower purity and a higher level of apoptosis than granulocytes isolated from the WB.

Conclusion

Compared with WB, the PBMCs isolated from the filters used in our blood center had no statistical difference in their activity and biological function, but they did differ in the proportion and quantity of monocytes and granulocytes. Our results show that the two filters can be used as an alternative method to collect leukocytes, which solves the problem of an insufficient blood supply for clinical and basic science research. Thus, these filters have significant value beyond their practical use in clinics.

中文翻译：

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两种白细胞过滤器分离的外周血单个核细胞的表征

目的

这项研究的目的是比较使用单采单采血小板白细胞减少系统室（LRSC），全血白细胞减少过滤器（LRF）和未过滤的外周全血（WB）中的白细胞分离的白细胞的活性和生物学功能。

方法

通过使用回收滤器和WB的密度梯度离心获得外周血单核细胞（PBMC）和粒细胞。流式细胞仪用于检测每种细胞亚型的活性，表型和凋亡率。

结果

与传统的分离方法相比，使用两种不同的滤膜时，从PBMC获得的淋巴细胞比例相似。然而，单核细胞和粒细胞之间存在显着差异。淋巴细胞的表型频率相似，但两个滤膜的淋巴细胞凋亡率略高。另外，通过三种来源分离的单核细胞能够被诱导成表达特定分子的树突状细胞。与从WB分离的粒细胞相比，从LRF分离的粒细胞显示出较低的纯度和较高的凋亡水平。

结论

与WB相比，从我们血液中心使用的滤器中分离出的PBMC的活性和生物学功能无统计学差异，但单核细胞和粒细胞的比例和数量却有差异。我们的结果表明，这两种过滤器可以用作收集白细胞的替代方法，从而解决了临床和基础科学研究中血液供应不足的问题。因此，这些过滤器具有超出其在临床中实际应用的重要价值。