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Efficiency of constructed wetlands in treating E . coli bacteria present in livestock wastewater
International Journal of Environmental Science and Technology ( IF 3.1 ) Pub Date : 2019-07-27 , DOI: 10.1007/s13762-019-02481-6
R. J. Rajan , J. S. Sudarsan , S. Nithiyanantham

Constructed wetlands are artificial wetland units, designed for adopting the principle of natural wetlands for purifying wastewater. Well-designed and well-constructed wetlands play an important role not only in reduction in organic compound/impurities, but also in coliform reduction in wastewater. The three small laboratory-scale units were control, Typha and plant species design based on USEPA procedure. The plant species are watered for a few days to promote their stable growth. Then, the three wetland units were seeded with livestock wastewater from a nearby cattle farm. Qualitative analysis by polymerase chain reaction and quantitative analysis are carried out by doing microbial culture in selective medium of MacConkey agar. The E. coli load is monitored before and after treatment in all the three units with different trials. It is found to be at a higher level in Typha unit after 24 h than in mixed and control units. The sample from the bottom of the constructed wetland unit is withdrawn and cultured for five days, and the bacterial load is found to be 9 × 104. Meanwhile, considerable reduction is observed in the Typha wetland unit after 5 days, and the bacterial load is 4 × 102. Mixed species wetland shows excellent treatment efficiency, and the bacterial load (E. coli) is very less after 5 days. It is evident from the study that constructed wetlands are effective in the treatment of E. coli bacteria wetland plants which are used for treatment.

中文翻译:

人工湿地处理大肠杆菌的效率。家畜废水中存在的大肠杆菌

人工湿地是人工湿地单元,旨在采用自然湿地原理净化废水。设计合理,结构合理的湿地不仅在减少有机化合物/杂质方面起着重要作用,而且在减少废水中大肠菌群方面也起着重要的作用。三个小型实验室规模的单位是对照,香蒲和基于USEPA程序的植物物种设计。将植物种浇水几天以促进其稳定生长。然后,在三个湿地单元中播种附近牧场的牲畜废水。通过在MacConkey琼脂的选择性培养基中进行微生物培养来进行聚合酶链反应的定性分析和定量分析。该大肠杆菌在所有三个单元的治疗前后,通过不同的试验监测负荷。发现在24小时后,香蒲单位的血脂水平高于混合和对照单位。从建造的湿地单元底部取出样品并培养五天,发现细菌载量为9×10 4。同时,在5天后,香蒲湿地单元观察到大量减少,细菌载量为4×10 2。混种湿地显示出优异的处理效率,以及细菌负荷(Ë大肠杆菌)是在5天后非常少。从研究中可以明显看出,人工湿地可以有效治疗E。用于治疗的大肠杆菌细菌湿地植物。
更新日期:2019-07-27
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