Abstract

Efficient expression of the man1 gene for Aspergillus aculeatus β-1,4-mannanase was demonstrated in Pichia pastoris yeast for the first time, and industrially important characteristics of the synthesized enzyme were confirmed. β-Mannanases are industrial enzymes that can be used, in particular, in the feed industry. The most important requirements for feed enzymes are a broad pH range, thermal stability, and high specific activity. The obtained data indicate that the man1 gene from A. aculeatus is potentially useful for the construction of industrial mannanase producers based on Pichia pastoris yeast.

中文翻译：

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曲霉β-甘露聚糖酶在毕赤酵母中的表达及其工业重要酶学性质的分析。

摘要

首次在巴斯德毕赤酵母中证实了刺曲霉β-1,4-甘露聚糖酶的man1基因的高效表达，并确认了该酶的工业重要特性。β-甘露聚糖酶是可特别用于饲料工业的工业酶。饲料酶的最重要要求是宽的pH范围，热稳定性和高比活度。获得的数据表明，来自棘孢曲霉的man1基因可能对构建基于巴斯德毕赤酵母的工业甘露聚糖酶生产者有用。