TGA family transcription factors are downstream components of the salicylic acid (SA) pathway and play a crucial role in the penetration defense against powdery mildew, a disease caused by the biotrophic pathogen Podosphaera aphanis that can cause enormous financial losses. However, the TGA genes in the octoploid strawberry (Benihoppe) remain largely unexplored. In this study, 11 FaTGA genes were identified and grouped into five subgroups based on the Strawberry GARDEN database. The evolution of TGAs has been relatively conservative, maintaining nearly the same protein domain and motif architectures. RT-qPCR showed that FaTGAs have different expression patterns in roots, stems, and leaves. The characteristics of their gene expression patterns indicate that FaTGA1, FaTGA2, FaTGA5, FaTGA7, FaTGA8, FaTGA9, and FaTGA10, as well as SA-responsive genes, were responsive to P. aphanis infection. In particular, exogenous SA induced significant upregulation of FaTGA and SA-responsive genes. Further correlation analysis suggested that FaTGA1, FaTGA2, FaTGA5, FaTGA7, FaTGA8, and FaTGA10 may play a crucial role in the response to powdery mildew disease. Taken together, our results reveal the responses of TGA genes in the strawberry P. aphanis interaction, which will provide further insight into the strawberry defense against powdery mildew.

中文翻译：

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八倍体草莓TGA基因的表达模式揭示了对Podosphaera aphanis感染的响应的潜在作用

TGA家族转录因子是水杨酸（SA）途径的下游组成部分，在抗白粉病的渗透防御中起着至关重要的作用，白粉病是由生物营养性病原体Podosphaera aphanis引起的，可导致巨大的经济损失。但是，八倍体草莓（Benihoppe）中的TGA基因仍未开发。在这项研究中，基于草莓GARDEN数据库，鉴定了11个FaTGA基因并将其分为五个亚组。TGA的进化相对保守，维持几乎相同的蛋白质结构域和基序结构。RT-qPCR显示FaTGA在根，茎和叶中具有不同的表达模式。它们的基因表达模式的特征表明，FaTGA1，FaTGA2，FaTGA5，FaTGA7，FaTGA8，FaTGA9和FaTGA10以及SA响应基因对潘氏假单胞菌感染有响应。特别是，外源性SA诱导FaTGA和SA响应基因显着上调。进一步的相关分析表明，FaTGA1，FaTGA2，FaTGA5，FaTGA7，FaTGA8和FaTGA10可能在白粉病的反应中起关键作用。两者合计，我们的结果揭示了草莓假单胞菌相互作用中TGA基因的反应，这将为草莓防御白粉病提供进一步的见解。