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Micropropagation of Three Commercial Cultivars of Hazelnut ( Corylus avellana  L.)
Gesunde Pflanzen ( IF 3.1 ) Pub Date : 2019-09-13 , DOI: 10.1007/s10343-019-00481-7
Neda Mardani , Ali Khadivi , Ali Vatanpour-Azghandi

The traditional methods of propagation of hazelnuts are time-consuming and not satisfactory at the commercial level. Therefore, the goal of current investigation was to optimize micropropagation of three commercial cultivars of hazelnut including ‘Gercheh’, ‘Gerdooyi’ and ‘Pashmineh’. In the present experiment, the effects of four levels of Benzylaminopurine (BAP; 0.50, 1.00, 2.00 and 4.00 mg L−1) with kinetin (0.25 mg L−1) and without kinetin on the shoot induction and proliferation were investigated. In addition, the effects of six treatments (NRM3 [control], NRM3/2, 0.50 mg L−1 IBA + 0.50 mg L−1 NAA, 0.50 mg L−1 IBA, 1.00 mg L−1 IBA + 1.00 mg L−1 NAA, and 0.50 mg L−1 NAA) on rooting of the studied cultivars were studied. Results showed that the best treatment for shoot proliferation for ‘Gercheh’ was 0.25 mg L−1 kinetin + 0.50 mg L−1 BAP as well as the best treatment for rooting of this cultivar was 1.00 mg L−1 IBA + 1.00 mg L−1 NAA. In ‘Gerdooyi’, the optimum treatment for shoot proliferation was 0.25 mg L−1 kinetin + 4.00 mg L−1 BAP, while the best treatment for its rooting was 0.50 mg L−1 IBA + 0.50 mg L−1 NAA. In ‘Pashmineh’, the best treatment for shoot proliferation was 0.00 mg L−1 kinetin + 0.50 mg L−1 BAP. In addition, the best treatment for rooting of this cultivar was NRM3/2. The results presented herein could be used for micropropagation of hazelnut.

中文翻译:

三种商业化榛子栽培品种(Corylus avellana L.)的微繁

榛子的传统繁殖方法很费时,在商业水平上并不令人满意。因此,当前研究的目的是优化三种商品榛子的微繁,包括“ Gercheh”,“ Gerdooyi”和“ Pashmineh”。在本实验中,研究了四种水平的苄基氨基嘌呤(BAP; 0.50、1.00、2.00和4.00 mg L -1),含激动素(0.25 mg L -1)和不含激动素对芽诱导和增殖的影响。另外,6个处理(NRM3 [控制],NRM3 / 2,0.50毫克的L的影响-1 IBA + 0.50毫克的L -1萘乙酸,0.50毫克的L -1 IBA,1.00毫克的L -1 IBA + 1.00毫克的L - 1个NAA和0.50 mg L -1 NAA)的生根研究。结果表明,芽增殖的最佳治疗关于“Gercheh”为0.25毫克的L -1激动素+ 0.50毫克的L -1 BAP以及用于该品种的生根最好的治疗为1.00毫克的L -1 IBA + 1.00毫克的L - 1 NAA。在“ Gerdooyi”中,芽增殖的最佳处理是0.25 mg L -1激动素+ 4.00 mg L -1 BAP,而生根的最佳处理是0.50 mg L -1 IBA + 0.50 mg L -1 NAA。在“ Pashmineh”中,芽增殖的最佳治疗方法是0.00 mg L -1激动素+ 0.50 mg L -1BAP。另外,使该品种生根的最佳方法是NRM3 / 2。本文介绍的结果可用于榛子的微繁。
更新日期:2019-09-13
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