Taxonomic confusion among island flying foxes (Pteropus hypomelanus) still in debate as in the lack of further genetic studies that describe subspecies validity needs verification. On the basis of morphological variants, 17 subspecies of P. hypomelanus are currently recognized. A previous study showed the distribution of two subspecies of P. hypomelanus in Malaysia: P. hypomelanus robinsoni in Pangkor Island, west coast of Peninsular Malaysia and P. hypomelanus lepidus from Perhentian Island, Kapas Island, and Tioman Island, east coast of Peninsular Malaysia. Herein, using the displacement loop (D-loop) region of mitochondrial DNA sequences, phylogenetic and haplotype network was conducted on their population structure in Peninsular Malaysia upon 42 genetic samples from Dangli Island (Langkawi), Pangkor Island (Perak), Redang Island (Terengganu), and Tinggi Island (Johor). Phylogenetically, two main clades separating northwest and southeast populations emerged. No haplotype was shared between the populations. The results from genetic differentiation (estimate of population subdivision (F_ST) and nucleotide subdivision (Nst)) yielded a gene flow that was nearly cut-off because of the central mountain that forms as an effective barrier between islands of the east and west coasts of Peninsular Malaysia. Although the above finding genetically confirms their subspecies designation, examination using a nuclear marker is needed in future studies.

中文翻译：

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利用线粒体DNA的D-环区确定岛飞狐的单倍型和网络分析，以确认亚种的命名

岛上狐狸（Pteropus hypomelanus）之间的分类混乱仍在争论中，因为缺乏描述亚种有效性的进一步的遗传学研究需要验证。根据形态变异，P的17个亚种。目前已认识到下肢黑色。先前的研究表明P的两个亚种的分布。马来西亚的下肢黑色：P。hypomelanus robinsoni在邦咯岛，马来西亚半岛和西海岸P。下睑下垂来自马来西亚半岛东海岸的Perhentian岛，Kapas岛和Tioman岛。在这里，利用线粒体DNA序列的置换环（D-loop）区域，对来自马来西亚槟城（Danggli Island）（兰卡威），邦咯岛（Perak），热浪岛（Redang Island）（登嘉楼）和丁宜岛（柔佛州）。从系统发育上讲，出现了将西北和东南种群分开的两个主要进化枝。种群之间没有共享单倍型。遗传分化的结果（人口细分估算（F _ST）和核苷酸细分（Nst）产生的基因流几乎被切断，因为中央山脉形成了马来西亚半岛东海岸和西海岸岛屿之间的有效屏障。尽管以上发现在遗传上证实了其亚种的指定，但在未来的研究中仍需要使用核标记进行检查。