Aspergillus flavipes FP-500 is a Mexican native strain that has been reported as a good producer of xylanases and pectinases; therefore, it promises a strong impact on biotechnology. To provide an overview of protein secretion by A. flavipes, we carried out a comparative proteome analysis of extracellular proteins in liquid cultures with two heterogeneous agro-industrial residues; corn cob (CC) and wheat bran (WB), as carbon sources. Extracellular proteins obtained from both cultures were identified using MS/MS spectrometry. We identified 134 proteins, which were classified into four groups: glycosyl hydrolases (GH), esterases/proteases, miscellaneous proteins, and unidentified proteins. Around 50% of the total proteins identified were GH such as xylanases, β-xylosidases, β-galactosidases, cellulolytic enzymes like β-glucosidase, endoglucanases, and cellobiohydrolases. From this family, a core of 22 (16%) of the proteins identified were found in both substrates, CC and WB, whereas 30% and 54% were unique for CC and WB, respectively. In the esterases/proteases group, proteases, lipases and esterases like feruloylesterases, and acetyl-xylanesterase were identified. Proteins with diverse functions such as monophosphate dehydrogenase or N-acetylglucosaminidase were present. Here, we present strong evidences indicating that the composition and heterogeneity of the used carbon source determine the specific set of protein secreted by the fungus.

黄曲霉FP-500是墨西哥本地菌株，据报道是木聚糖酶和果胶酶的良好生产者。因此，它有望对生物技术产生巨大影响。概述黄曲霉分泌的蛋白质，我们对带有两个异质农工残基的液体培养物中的细胞外蛋白质进行了蛋白质组比较分析；玉米芯（CC）和麦麸（WB）作为碳源。使用MS / MS光谱法鉴定了从两种培养物中获得的细胞外蛋白。我们鉴定了134种蛋白质，分为四类：糖基水解酶（GH），酯酶/蛋白酶，其他蛋白质和未鉴定的蛋白质。鉴定的总蛋白质中约有50％是GH，例如木聚糖酶，β-木糖苷酶，β-半乳糖苷酶，纤维素分解酶（例如β-葡萄糖苷酶），内切葡聚糖酶和纤维二糖水解酶。在这个家族中，在CC和WB两种底物中均发现了22种（16％）已鉴定蛋白的核心，而CC和WB分别具有30％和54％的独特性。在酯酶/蛋白酶组中，蛋白酶 脂肪酶和酯酶，如阿魏酸酯酶和乙酰木聚糖酯酶。存在具有多种功能的蛋白质，例如单磷酸脱氢酶或N-乙酰氨基葡糖苷酶。在这里，我们提供有力的证据表明所用碳源的组成和异质性决定了真菌分泌的特定蛋白质组。