In the present study, phylogenetic relationships within Heterobranchia in particular to Pulmonata were evaluated by means of Histone-3 (H3) gene sequence information. H3 gene is a slow evolving marker and is useful in resolving the deep level relationships. This is the first study to report the phylogeny of Pulmonata with more number of representatives from the group on the basis of H3 gene. The major groups within Heterobranchia viz. Lower Heterobranchia, Opisthobranchia, and Pulmonata were non-monophyletic. A few of the pulmonate groups’ viz. Planorbidae, Lymnaeidae, Siphonariidae, Veronicellidae, and Stylommatophora were recovered as monophyletic. The concepts of Eupulmonata and Geophila were not observed in the present study. The present study was undertaken with an objective to study the phylogeny of Pulmonata reconstructed on the basis of H3 gene and its ability to resolve the deeper divergences in Pulmonata. However, the resolution at the deeper nodes is limited. There is a good resolution at the level of genera. In the future, inclusion of more number of taxa with increased sequence length of H3 marker may yield resolved topologies that may shed more insights into the phylogeny of Pulmonata.

中文翻译：

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基于 Histone-3 基因的 Pulmonata (Mollusca: Gastropoda) 分子系统发育分析

在本研究中，通过组蛋白 3 (H3) 基因序列信息评估了异鳃类动物内的系统发育关系，特别是与 Pulmonata 的系统发育关系。H3 基因是一个缓慢进化的标记，可用于解决深层关系。这是第一项以H3基因为基础报道Pulmonata系统发育的研究，具有更多的代表数量。Heterobranchia 中的主要群体即。下异鳃纲、后鳃纲和肺纲是非单系的。一些肺组织的即。Planorbidae、Lymnaeidae、Siphonariidae、Veronicellidae 和 Stylommatophora 作为单系被回收。在本研究中没有观察到 Eupulmonata 和 Geophila 的概念。本研究旨在研究基于 H3 基因重建的 Pulmonata 的系统发育及其解决 Pulmonata 更深层次分歧的能力。然而，更深节点的分辨率是有限的。在属水平上有很好的分辨率。将来，包含更多数量的具有增加 H3 标记序列长度的分类群可能会产生已解析的拓扑结构，这可能会为 Pulmonata 的系统发育提供更多见解。