Abstract This study was conducted to fabricate scaffold from polylactic acid (PLA) and hydroxyapatite (HA) extracted from waste bovine bone for enhancing both mechanical and biocompatible properties. After pretreatment in dilute NaOH solution, the bone was calcined at 900°C for 6 h, ball milled and converted to HA. Factors that affect the formation of HA were investigated. Experimental results showed that HA particles with crystal size < 100 nm and 99% crystallinity could be obtained at 90°C, pH 11 and 35 mM H3PO4 solution followed by 4 h calcination at 900°C. By using non-solvent induced phase separation method, PLA scaffolds with pore size and surface area of 22.6 μm and 25.7 m2/g, respectively, containing different hydroxyapatite were successfully prepared. Tensile strength of scaffolds increased due to effective support by HA grafted collagen. PLA scaffolds containing HA were more degradable than PLA scaffolds and PLA scaffolds containing HA grafted collagen. Cell culture results showed that cell density increased significantly on porous scaffolds than that on the dense scaffolds. Moreover, cell density also increased significantly on the scaffold containing HA grafted collagen than that on the scaffold with pure HA.

中文翻译：

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从废牛骨中提取的羟基磷灰石制备生物复合支架

摘要 本研究旨在从废牛骨中提取的聚乳酸 (PLA) 和羟基磷灰石 (HA) 制备支架，以提高机械和生物相容性。在稀 NaOH 溶液中预处理后，将骨头在 900°C 下煅烧 6 小时，球磨并转化为 HA。研究了影响HA形成的因素。实验结果表明，在 90°C、pH 11 和 35 mM H3PO4 溶液，然后在 900°C 下煅烧 4 小时，可以获得晶体尺寸 < 100 nm 和 99% 结晶度的 HA 颗粒。采用非溶剂诱导相分离方法，成功制备了孔径和表面积分别为22.6 μm和25.7 m2/g、含有不同羟基磷灰石的PLA支架。由于 HA 接枝胶原的有效支撑，支架的拉伸强度增加。含有 HA 的 PLA 支架比含有 HA 接枝胶原蛋白的 PLA 支架和 PLA 支架更易降解。细胞培养结果表明，多孔支架上的细胞密度比致密支架上的细胞密度显着增加。此外，含有 HA 移植胶原蛋白的支架上的细胞密度也比含有纯 HA 的支架上的细胞密度显着增加。