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Knockdown of cadherin genes decreases susceptibility of Chilo suppressalis larvae to Bacillus thuringiensis produced Crystal toxins.
Insect Molecular Biology ( IF 2.6 ) Pub Date : 2020-01-21 , DOI: 10.1111/imb.12634
H Zhou 1, 2 , W Hu 2 , Q Huang 2 , M Abouzaid 2 , H Jin 2 , Y Sun 2 , L Qiu 3 , W Zhang 1 , Y Lin 1 , W Ma 1, 2
Affiliation  

The striped rice stem borer, Chilo suppressalis Walker, is one of the most destructive rice pests in Asia. Insecticidal crystal proteins (Cry toxins) produced by Bacillus thuringiensis are widely used as biopesticides or in developing transgenic crops for pest management. In this study, we tested the involvement of two newly cloned C. suppressalis cadherins (CsCAD3 and CsCAD4) in the toxicity of Cry1Ab/Ac, Cry2Aa and Cry1Ca. Our results showed that CsCAD4 was expressed highest in the midgut, whereas CsCAD3 was expressed highest in the epidermis. The feeding of double‐stranded RNA specific to CsCAD3 and CsCAD4 respectively significantly suppressed the expressions of target gene. The knockdown of CsCAD3 significantly reduced the mortality of larvae to Cry1Ab/Ac, whereas knockdown of CsCAD4 significantly decreased the larval susceptibility to Cry2Aa. In contrast, reduced expressions of CsCAD3 or CsCAD4 were not interacted with larval susceptibility to Cry1Ca. Our results suggest that CsCAD3 and CsCAD4 function in Cry toxin toxicity and these findings will help us to better understand the action mechanism of Cry toxins in C. suppressalis.

中文翻译:

敲低钙黏着蛋白基因降低了Chilohibialis幼虫对苏云金芽孢杆菌产生的晶体毒素的敏感性。

条纹水稻stem虫Chilo inhibitoralis Walker是亚洲最具破坏性的水稻害虫之一。苏云金芽孢杆菌产生的杀虫晶体蛋白(Cry毒素)被广泛用作生物农药或用于开发用于害虫管理的转基因作物。在这项研究中,我们测试了两个新克隆的抑制叶梭菌钙粘蛋白(CsCAD3CsCAD4)与Cry1Ab / Ac,Cry2Aa和Cry1Ca的毒性有关。我们的结果表明,CsCAD4在中肠的表达最高,而CsCAD3在表皮的表达最高。CsCAD3CsCAD4特异的双链RNA的进料分别显着抑制了靶基因的表达。击倒CsCAD3显着降低了幼虫对Cry1Ab / Ac的死亡率,而击倒CsCAD4则显着降低了幼虫对Cry2Aa的敏感性。相反,CsCAD3CsCAD4的降低表达与幼虫对Cry1Ca的敏感性无关。我们的结果表明,CsCAD3CsCAD4在Cry毒素毒性中起作用,这些发现将有助于我们更好地了解C.毒素在抑制中的作用机制。
更新日期:2020-01-21
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