The review presents a chronological development of procedures for determining the concentrations of total protein and albumin; total concentration of globulins; concentrations of subfractions, classes, and subclasses of globulins; and total concentrations of globulin subclasses in human blood. A brief overview of these procedures and the results of their comparative tests in medical examination of patients are given. The considered procedures are systematized; their advantages and disadvantages are discussed. It is noted that procedures for measuring the concentration of total protein, albumin, and globulin classes in plasma, serum, and whole blood are widely used in modern clinical diagnostic laboratories when performing routine assays. The concentrations of globulin subclasses and total concentrations of globulin subclasses in blood are mainly determined in biomedical research. Procedures for measuring the concentration of total globulins and globulin subfractions in plasma and serum are of historical importance and now are barely applied in clinical and diagnostic laboratories.

中文翻译：

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血液中蛋白质的测定。第1部分：总蛋白和白蛋白的测定

审查提出了确定总蛋白和白蛋白浓度的程序的时间顺序发展；球蛋白的总浓度；球蛋白的亚组分，类别和亚类的浓度；和人类血液中球蛋白亚类的总浓度。简要概述了这些程序及其在患者体检中的比较测试结果。考虑的程序已系统化；讨论了它们的优缺点。应当指出，在进行常规测定时，用于测量血浆，血清和全血中总蛋白，白蛋白和球蛋白类别浓度的程序已广泛用于现代临床诊断实验室。血液中球蛋白亚类的浓度和球蛋白亚类的总浓度主要在生物医学研究中确定。测量血浆和血清中总球蛋白和球蛋白亚组分浓度的方法具有历史意义，目前在临床和诊断实验室中几乎没有应用。