Grifola frondosa is a basidiomycete fungus with potential biomedical applications owing to the presence of bioactive polysaccharides. The activities of polysaccharides are influenced by many factors, particularly temperature; however, the optimal temperature and conditions for preparation of polysaccharides from this organism have not yet been determined. Therefore, in this study, cold-water soluble polysaccharides from Grifola frondosa were extracted at 4 °C (GFP-4) and purified. GFP-4-30, GFP-4-60 and GFP-4-90 were obtained from GFP-4 after treatment at 30 °C, 60 °C, or 90 °C, respectively, for 6 h. MTT results showed that GFP-4 had the highest inhibitory effects on the proliferation of SPC-A-1 cells in vitro. High-performance gel permeation chromatography results demonstrated that the molecular weight of GFP-4 was 1.05 × 10⁶ Da and that GFP-4-30, GFP-4-60, and GFP-4-90 showed different levels of degradation and generated small molecule sugars. Fourier transform infrared spectroscopy, gas chromatography, and nuclear magnetic resonance results indicated that GFPs mainly consisted of α-d-Galp, α-d-Manp and α-d-Glcp. Periodate oxidation, Smith degradation, and methylation results showed that the backbones of the molecules consisted of 1,3-linked-Galp. After heat treatment, percentages of (1 → 3,4) α-d-Galp in heat-treated polysaccharides were obviously decreased, indicating their lower branching degree, and resulting in weaker antitumor effects. Overall, our findings demonstrated changes in the structure-activity relationships of GFP-4 after heat treatment and provided a theoretical basis for the application of GFP-4 in the food and drug industries.

中文翻译：

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热处理对灰树花冷水溶性多糖的结构性质与抗肿瘤活性的关系

Grifola frondosa是一种担子菌真菌，由于存在生物活性多糖，具有潜在的生物医学用途。多糖的活性受许多因素的影响，特别是温度。然而，尚未确定从该生物体制备多糖的最佳温度和条件。因此，在本研究中，在4°C下提取了Grifola frondosa的冷水溶性多糖（GFP-4）并进行了纯化。在分别于30°C，60°C或90°C处理6小时后，从GFP-4获得GFP-4-30，GFP-4-60和GFP-4-90。MTT结果表明，GFP-4对SPC-A-1细胞的体外增殖抑制作用最高。。高效凝胶渗透色谱分析结果表明，GFP-4的分子量为1.05×10 ⁶ Da，GFP-4-30，GFP-4-60和GFP-4-90的降解程度不同，并且生成量很小。分子糖。傅里叶变换红外光谱，气相色谱法，和核磁共振结果表明的GFP主要由α-的d -Gal p，α- d -Man p和α- d -Glc p。高碘酸盐的氧化，Smith降解和甲基化的结果表明，分子的主链由1,3-连接的Gal P组成。热处理后，（1→3,4）α- d -Gal的百分比热处理过的多糖中的p明显降低，表明它们的分支度较低，从而导致较弱的抗肿瘤作用。总体而言，我们的发现证明了热处理后GFP-4的构效关系的变化，并为GFP-4在食品和药物工业中的应用提供了理论基础。