Marker-assisted selection (MAS) is becoming increasingly common in commercial potato breeding programmes and can provide information to breeders on potential resistance to diseases and pests early in the breeding cycle when phenotypic screens are impractical. In recent years, a wealth of information on molecular markers for genes involved in resistance to biotic stresses of potato has been revealed. However, markers come in many different forms and ideally a breeding programme would routinely perform annual genotyping on a single unified platform. In this study, we present an approach to consolidate various types of molecular markers to a single SNP-based platform using whole-genome sequencing of carefully constructed pools of DNA using, as examples, molecular markers for the H1 nematode and R2 blight resistance genes. The approach led to the identification of a number of candidate nucleotide polymorphisms which allowed successful design and deployment of kompetitive allele-specific PCR (KASP) assays. We further show how the sequence data generated can be used to aid design of KASP assays for diagnostic SNPs for other traits mined from the literature.

中文翻译：

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使用汇集的全基因组重测序快速开发抗病基因的 KASP 标记

标记辅助选择 (MAS) 在商业马铃薯育种计划中变得越来越普遍，当表型筛选不切实际时，它可以在育种周期的早期向育种者提供有关病虫害潜在抗性的信息。近年来，大量有关马铃薯生物胁迫抗性基因分子标记的信息被揭示。然而，标记有许多不同的形式，理想情况下，育种计划将在一个统一的平台上定期进行年度基因分型。在本研究中，我们提出了一种方法，使用 H1 线虫和 R2 抗疫基因的分子标记，对精心构建的 DNA 池进行全基因组测序，将各种类型的分子标记整合到一个基于 SNP 的平台上。该方法导致了许多候选核苷酸多态性的鉴定，这使得竞争性等位基因特异性 PCR (KASP) 检测的成功设计和部署成为可能。我们进一步展示了如何使用生成的序列数据来帮助设计用于从文献中挖掘的其他性状的诊断 SNP 的 KASP 检测。