Brassica juncea is one of important oilseed crops, and FA compositions determine quality of vegetable oil. Although fatty acid desaturases (FADs) are mainly responsible for modifying seed FA compositions, genome-wide analysis of FAD gene family in B. juncea (BjuFAD) is not reported. Here, we identified 57 BjuFAD genes in B. juncea genome using homology searches. These FAD genes were unevenly distributed in 16 chromosomes and 3 scaffolds. Phylogenetic analysis showed that BjuFAD genes were divided into seven subfamilies. Exon–intron organizations, intron patterns and MEME motifs were highly conserved within each of BjuFAD subfamilies. Moreover, subcellular locations of deduced BjuFAD proteins and cis-acting elements in BjuFAD promoters were predicted and analyzed using online software. In addition, 16 SSR loci were totally found in BjuFAD genes/promoters. This work provides a basis for further function study of BjuFAD genes in quality improvement of B. juncea seed oil and in plant development as well as stress response.

中文翻译：

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芥菜中FAD基因家族的全基因组挖掘和计算机分析

芥菜是重要的油料作物之一，FA的成分决定了植物油的品质。尽管脂肪酸去饱和酶（FADs）主要负责修饰种子FA的组成，但尚无关于芥菜型杆菌（BjuFAD）中FAD基因家族的全基因组分析的报道。在这里，我们使用同源搜索在芥菜芽孢杆菌基因组中鉴定了57个BjuFAD基因。这些FAD基因分布在16个染色体和3个支架中。系统发育分析表明，BjuFAD基因被分为七个亚家族。外显子-内含子组织，内含子模式和MEME基序在每个BjuFAD中高度保守亚科。此外，使用在线软件预测并分析了BjuFAD启动子中推导的BjuFAD蛋白和顺式作用元件的亚细胞位置。此外，在BjuFAD基因/启动子中共发现16个SSR基因座。这项工作为进一步研究BjuFAD基因在芥菜油菜籽油的品质改善以及植物发育以及逆境响应中的功能提供了基础。