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Development of SSR Markers for Coconut ( Cocos nucifera L.) by Selectively Amplified Microsatellite (SAM) and Its Applications
Tropical Plant Biology ( IF 2 ) Pub Date : 2018-12-14 , DOI: 10.1007/s12042-018-9215-1
Yi Wu , Yaodong Yang , Rashad Qadri , Amjad Iqbal , Jing Li , Haikuo Fan , Yaoting Wu

Coconut is an important tropical fruit and oil crop. Its long generation time, low multiplication rate and recalcitrant seeds make coconut more difficult for breeding and selection. New technologies and extensive resources to evaluate coconut breeding strategies are the demand of today. Molecular markers had the potential to dramatically increase the efficiency and efficacy in the areas of germplasm management, genotype identification and marker-assisted selection of economically important traits of coconut. However, the lack of relevant molecular techniques impedes the development of a new strategy for the genetic improvement of the coconut. In this study, we have successfully developed 84 SSR markers by Selectively Amplified Microsatellite from coconut genome and more than 90% of these SSR showed good transferability to the palm family. The study will enrich the genomic-SSR pool for coconut and also for the other palm tree. Besides, it will provide the scientists with more options for coconut germplasm evaluation, constructing a coconut genetic linkage map and designing the breeding programs for producing superior cultivars of coconut.

中文翻译:

选择性扩增微卫星技术开发椰子SSR标记及其应用

椰子是重要的热带水果和油料作物。它的生成时间长,繁殖率低和顽强的种子,使椰子更难以繁殖和选择。当今,需要新技术和广泛的资源来评估椰子育种策略。在椰子的种质管理,基因型鉴定和标记辅助选择方面,分子标记具有极大地提高效率和功效的潜力。但是,相关分子技术的缺乏阻碍了椰子遗传改良新策略的发展。在这项研究中,我们已经通过选择性扩增微卫星从椰子基因组成功开发了84个SSR标记,其中超过90%的SSR显示出了向棕榈科的良好转移性。该研究将丰富用于椰子和其他棕榈树的基因组SSR库。此外,它将为科学家提供更多的椰子种质评估选择,构建椰子遗传连锁图谱,并设计育种程序,以生产出优质的椰子品种。
更新日期:2018-12-14
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