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Chromolaena odorata extract as a green agent for the synthesis of Ag@AgCl nanoparticles inactivating bacterial pathogens
Chemical Papers ( IF 2.2 ) Pub Date : 2019-12-20 , DOI: 10.1007/s11696-019-01033-z
Dien-Trung Nguyen , Nhat-Linh Duong , Van-Minh Nguyen , Cam-Van Thi Luong , Phung-Anh Nguyen , Tri Nguyen

In this work, the green synthesis of silver-silver chloride nanoparticles (Ag@AgClNPs) has been used the plant leaf extract of Chromolaena odorata (C. odorata) as a reducing and capping agent. The effects of parameters on the formation of Ag@AgCl nanoparticles (Ag@AgClNPs such as the ratio of C. odorata raw material/water, the extraction temperature, the extraction duration, and the volume ratios of AgNO3 solution and C. odorata extract have been studied. The obtained Ag@AgClNPs sample was characterized by ultraviolet visible spectroscopy, X-ray diffraction spectroscopy, Fourier transform infrared spectroscopy, energy-dispersive X-ray spectroscopy, and transmission electron microscopy. UV–visible absorption studies revealed surface plasmon resonance peak around 420‒450 nm, confirming the presence of Ag@AgClNPs. The average particle size was approximately 15.2 nm. The antibacterial activity of Ag@AgClNPs was carried out by the agar well diffusion method and the determination of the minimum inhibitory concentration that showed high toxicity against the gram-negative bacteria (Escherichia coli, Salmonella typhi, and Pseudomonas aeruginosa) and the gram-positive bacteria (Bacillus cereus and Staphylococcus aureus).

中文翻译:

香樟提取物作为绿色试剂,用于灭活细菌病原体的Ag @ AgCl纳米颗粒的合成

在这项工作中,银-氯化银纳米粒子(Ag @ AgClNPs)的绿色合成已被用作香樟木C. odorata)的植物叶片提取物,作为还原剂和加帽剂。的参数上形成的Ag @氯化银纳米颗粒的影响(AG @ AgClNPs如比例C.飞机草原料/水,提取温度,提取时间,和硝酸银的体积比3溶液和C.飞机草提取物已被研究。通过紫外可见光谱,X射线衍射光谱,傅立叶变换红外光谱,能量色散X射线光谱和透射电子显微镜对所得的Ag @ AgClNPs样品进行表征。紫外可见吸收研究表明,表面等离振子共振峰在420‒450 nm附近,证实了Ag @ AgClNPs的存在。平均粒径约为15.2nm。Ag @ AgClNPs的抗菌活性通过琼脂井扩散法进行,并确定对革兰氏阴性菌(大肠杆菌鼠伤寒沙门氏菌铜绿假单胞菌)及革兰氏阳性菌具有高毒性的最小抑菌浓度。细菌(蜡状芽孢杆菌金黄色葡萄球菌)。
更新日期:2019-12-20
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