Production of xylitol from lignocellulosic biomass is of interest to modern biorefineries, because this biomass should be processed into a spectrum of chemicals (bio-based products) and not only energy. The isolation of new yeast strains capable of efficiently converting xylose into xylitol and withstanding inhibitors released from biomass hydrolysis can contribute to making its production feasible in biorefineries. Forty-three out of 128 yeast strains isolated from the gut of Passalidae beetles were capable of assimilating xylose as the sole carbon source. Meyerozyma guilliermondii UFV-1 was selected due to its ability to grow and ferment D-xylose in a synthetic medium. This yeast assimilated the broad range of sugars present in lignocellulosic biomass hydrolysates, such as xylose, raffinose, cellobiose, rhamnose, arabinose, and glucose. Its optimum growth conditions were pH 8.0 and a temperature of 30 °C. In concentrations of 0.07 mol/L acetic acid, 0.05 mol/L 5-hydroximethylfurfural, and 0.04 mol/L furfural, M. guilliermondii UFV-1 did not grow. Maximum xylitol production in aerobiosis and hypoxia were 51.88 and 27.73 g/L, respectively. Under aerobic condition, xylose concentration and agitation rate were the factors which were statistically significant, while only the agitation rate was significant in hypoxia. We fitted a response surface (RS) that estimated the best agitation rate (113.33 rpm) and xylose concentration (90 g/L) for maximum xylitol production in aerobiosis. Therefore, M. guilliermondii UFV-1 displays potential for being used for xylitol production in biorefineries.

中文翻译：

---

木糖同化酵母的分离和新型Meyerozyma guilliermondii菌株对木糖醇生产的优化。

现代生物精炼厂对从木质纤维素生物质生产木糖醇很感兴趣，因为这种生物质应不仅加工成能源，而且应加工成多种化学品（基于生物的产品）。能够有效地将木糖转化为木糖醇并能承受生物质水解释放的抑制剂的新酵母菌株的分离，可以使其在生物精炼厂的生产变得可行。从Passalidae甲虫的肠道中分离出的128种酵母菌株中，有43种能够吸收木糖作为唯一碳源。瓜叶菌选择UFV-1是因为它具有在合成培养基中生长和发酵D-木糖的能力。该酵母吸收了木质纤维素生物质水解物中存在的多种糖，例如木糖，棉子糖，纤维二糖，鼠李糖，阿拉伯糖和葡萄糖。它的最佳生长条件是pH 8.0和温度30°C。浓度为0.07 mol / L的乙酸，0.05 mol / L的5-羟甲基糠醛和0.04 mol / L的糠醛UFV-1没有生长。需氧量和低氧状态下的最大木糖醇产量分别为51.88和27.73 g / L。在有氧条件下，木糖浓度和搅拌速率是统计学上显着的因素，而在低氧条件下，只有搅拌速率才是显着的。我们安装了一个响应面（RS），该响应面估计了最佳的搅动速率（113.33 rpm）和木糖浓度（90 g / L），以使氧合生物中木糖醇的产量最高。因此，Guilliermondii UFV-1具有在生物精炼厂生产木糖醇的潜力。