Cryopreservation is a useful approach to preserve male fertility for assisted reproduction technique and other evaluations. In spite of extensive development in the cryopreservation field, there are biological and biochemical points including DNA fragmentation and antioxidant which should be illuminated to preserve fertility in safe form. Several molecular markers such as coding and noncoding RNAs are transferred from spermatozoa into oocyte via fertilization. These biomarkers affect fertility potential during the cryopreservation. Despite its impact, sperm cryopreservation has some destructive effect including loss of sperm motility and viability, acrosomal damage, mitochondrial membrane depolarization, plasma membrane permeability alteration even nuclear, and DNA damage. There is a growing concern about the impact of sperm cryopreservation on biological factors which can interrupt successful fertility procedures such as in vitro fertilization. Additionally, cryo-damage can decrease embryonic development extent. Promoting cryopreservation method via investigating the wide range of non-invasive factors may be increasingly important to have access to safe approach of freezing. Therefore, the aim of this study is the assessment of biological factors which can penetrate the fertility potential during the freezing procedure, explicate innovative methods in fertility preservation.

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冷冻保存对哺乳动物精子结构和功能的影响：概述

冷冻保存是保存男性生殖力的有用方法，可用于辅助生殖技术和其他评估。尽管在冷冻保存领域进行了广泛的开发，但仍有一些生物和生化点，包括DNA片段化和抗氧化剂，应加以照明以安全地保存生育力。几种分子标记（例如编码和非编码RNA）通过受精从精子转移到卵母细胞中。这些生物标记物会影响冷冻保存过程中的生育能力。尽管有其影响，但精子冷冻保存仍具有一定的破坏作用，包括精子活力和活力丧失，顶体损伤，线粒体膜去极化，质膜通透性改变，甚至核和DNA损伤。人们越来越关注精子冷冻保存对可能中断成功的生育程序（例如体外受精）的生物学因素的影响。另外，冷冻损伤会降低胚胎的发育程度。通过研究广泛的非侵入性因素来促进冷冻保存方法对于获得安全的冷冻方法可能越来越重要。因此，本研究的目的是评估在冷冻过程中可以穿透生育潜能的生物学因素，从而阐明保护生育力的创新方法。通过研究广泛的非侵入性因素来促进冷冻保存方法对于获得安全的冷冻方法可能越来越重要。因此，本研究的目的是评估在冷冻过程中可以穿透生育潜能的生物学因素，从而阐明保护生育力的创新方法。通过研究广泛的非侵入性因素来促进冷冻保存方法对于获得安全的冷冻方法可能越来越重要。因此，本研究的目的是评估在冷冻过程中可以穿透生育潜能的生物学因素，从而阐明保护生育力的创新方法。