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Multiple novel hepatocellular carcinoma signature genes are commonly controlled by the master pluripotency factor OCT4
Cellular Oncology ( IF 6.6 ) Pub Date : 2019-12-17 , DOI: 10.1007/s13402-019-00487-3 Chao Ye 1, 2 , Xiaoqian Zhang 1 , Xinyu Chen 1 , Qingyi Cao 1 , Xiaobing Zhang 1 , Yanwen Zhou 1, 3 , Wenxin Li 1, 4 , Liangjie Hong 5, 6 , Haiyang Xie 5, 6 , Xiaoli Liu 1 , Hongcui Cao 1 , Ying-Jie Wang 1 , Bo Kang 1
中文翻译:
多个新的肝细胞癌特征基因通常由多能性主因子OCT4控制
更新日期:2019-12-17
Cellular Oncology ( IF 6.6 ) Pub Date : 2019-12-17 , DOI: 10.1007/s13402-019-00487-3 Chao Ye 1, 2 , Xiaoqian Zhang 1 , Xinyu Chen 1 , Qingyi Cao 1 , Xiaobing Zhang 1 , Yanwen Zhou 1, 3 , Wenxin Li 1, 4 , Liangjie Hong 5, 6 , Haiyang Xie 5, 6 , Xiaoli Liu 1 , Hongcui Cao 1 , Ying-Jie Wang 1 , Bo Kang 1
Affiliation
Abstract
Background
Worldwide, hepatocellular carcinoma (HCC) is a common solid tumor with a poor prognosis. HCC is often due to hepatitis B virus (HBV) infection. As yet, efficacious HCC treatment regimens for late-stage HCC patients are lacking. Therefore, the identification of more specific and sensitive biomarkers for its early diagnosis and treatment remains an urgent need.Methods
Total RNAs from paired HBV-derived HCC tumors and adjacent peritumor tissues (APTs) were subjected to RNA sequencing (RNA-seq), and differentially expressed genes (DEGs) between HCC tumors and APTs were selected and verified.Results
We identified 166 DEGs and found that eight top-ranked and verified DEGs (TK1, CTTN, CEP72, TRIP13, FTH1, FLAD1, CHRM2, AMBP) all contained putative OCT4 binding motifs in their promoter regions. TK1, TRIP13 and OCT4 were found to exhibit concurrent higher expression levels in HCC tumors than in APTs. The mRNA levels of TK1, TRIP13 and OCT4 in a cohort of 384 HCC samples from the TCGA database were all found to be negatively correlated with patient overall survival, relapse-free survival and progression-free survival, underscoring the HCC biomarker status of TK1 and TRIP13 on one hand, and implicating their association with OCT4 on the other hand. Furthermore, OCT4 proteins were found to bind to the promoters of both genes in vitro and in vivo. Knocking out OCT4 in HCC-derived cell lines reduced the expression of TK1 and TRIP13 and significantly decreased their tumorigenicity.Conclusions
Using RNA-seq, we identified several novel HCC signature genes that may serve as biomarkers for its diagnosis and prognosis. Their common transcriptional regulation by OCT4 suggests key roles in the development of HCC, and indicates that OCT4 may serve as a potential therapeutic target.中文翻译:
多个新的肝细胞癌特征基因通常由多能性主因子OCT4控制