The complex of cobalt(III) ethylenediamine was synthesized, isolated and characterized by UV-Vis, IR, and 1H NMR spectral methods. The binding of the complex with calf thymus DNA was investigated by absorption and emission spectroscopy, viscosity measurements, DNA melting and DNA photocleavage. The spectroscopic studies together with the viscosity measurements and DNA melting studies indicated that the complex binds to calf thymus DNA in a nonintercalative mode. This complex is found to promote photocleavage of the DNA plasmid pBR322 and shows a cytotoxic effect against CHO cells.

中文翻译：

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钴（III）乙二胺吡唑配合物[Co（en）2（pyz）2] 3+的DNA结合和细胞毒性

合成，分离并通过UV-Vis，IR和1H NMR光谱表征了乙二胺钴（III）的配合物。通过吸收和发射光谱，粘度测量，DNA熔解和DNA光裂解研究了该复合物与小牛胸腺DNA的结合。光谱研究以及粘度测量和DNA熔解研究表明，该复合物以非插入模式与小牛胸腺DNA结合。发现该复合物促进DNA质粒pBR322的光裂解，并显示出对CHO细胞的细胞毒性作用。