Verticillium wilt causes substantial economic losses in eggplant by reducing its yield and quality. However, the relationship between the host and fungal pathogen is poorly understood. Large amounts of high quality clean reads were obtained from our samples. We used bioinformatics analysis to detect 373 differentially expressed miRNAs and 18 differentially expressed novel miRNAs. Eleven highly enriched MIRNA families were found to be involved in the resistance to Verticillium Wilt in eggplant. We identified the differentially expressed miRNAs among samples and analyzed their 78 target genes. These target genes were annotated with GO function enrichment and KEGG pathway association. The target genes included transcription factors such as TCP, E3 and FEX6 etc. Functional categorization of those target genes reflected a number of important pathways, which are involved in the plant’s resistance to various pathogens. These differentially expressed miRNAs and their target genes will provide a theoretical basis for the further study of Verticillium wilt-related miRNAs and the regulation of disease resistance mechanisms of eggplant at the miRNA level.

中文翻译：

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茄科植物华氏小RNA的深度测序分析

黄萎病会降低茄子的产量和质量，从而给茄子造成巨大的经济损失。但是，宿主和真菌病原体之间的关系知之甚少。从我们的样品中获得了大量高质量的纯净读数。我们使用生物信息学分析来检测373个差异表达的miRNA和18个差异表达的新型miRNA。发现11个高度富集的MIRNA家族与茄子对黄萎病的抗性有关。我们在样品中鉴定了差异表达的miRNA，并分析了它们的78个靶基因。这些靶基因用GO功能富集和KEGG途径结合进行注释。目标基因包括转录因子，例如TCP，E3和FEX6等。这些目标基因的功能分类反映了许多重要途径，这与植物对各种病原体的抗性有关。这些差异表达的miRNA及其靶基因将为进一步研究黄萎病相关的miRNA以及在miRNA水平上调控茄子的抗病机理提供理论依据。