The study was conducted to evaluate the effects of 10 and 20 min seed soaking in Azotobacter chroococcum and Bacillus polymixa suspension culture on seed germination, seedling growth and essential oil yield of Dracocephalum kotschyi Boiss. Up to day 13, 20 min of B. polymixa suspension culture soaking seeds had shown germination more than 20%, whereas control seeds did not begin germination until day 13. The highest germination rate (1.67) was observed with 20 min of B. polymixa suspension culture soaking seeds. B. polymixa improved seedling length and vigour index more than those of A. chroococcum suspension culture soaking seeds. The highest fresh (81.06 g) and dry weight (11.76 g), relative leaf chlorophyll content (42.24) and Fv/Fm ratio (0.66) of emerging D. Kotschyi seedlings were recorded with seed bio-priming with B. polymixa for 20 min. The main components affected by B. polymixa and A. chroococcum were limonene, α‑pinene, γ‑terpinene, caryophyllene oxide, germacrene D and terpinen-4-ol. The highest percentage of essential oil constituents (97.6%) of D. Kotschyi seedlings were observed with 20 min of B. polymixa suspension culture soaking seeds. The results indicated that seed bio-priming with B. polymixa for 20 min was more effective than the other treatments concerning seed germination, seedling growth and essential oil yield of D. Kotschyi.

中文翻译：

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种子生物引发可改善伊朗濒危药用植物Dracocephalum Kotschyi Boiss的发芽，幼苗生长和精油产量

进行了这项研究，以评估在蓝细菌和多芽芽孢杆菌悬浮培养物中浸泡10分钟和20分钟后，对Dracocephalum kotschyi Boiss种子发芽，幼苗生长和精油产量的影响。直到第13天，浸泡种子的B. polymixa悬浮培养物20分钟显示出超过20％的发芽率，而对照种子直到第13天才开始发芽。20分钟的polymixa混合芽孢杆菌观察到最高发芽率（1.67）。悬浮培养浸种。B. polymixa比A. chroococcum改善了幼苗的长度和活力指数悬浮培养浸种。用混合芽孢杆菌（B. polymixa）生物引发20分钟记录了新兴D. Kotschyi幼苗的最高新鲜（81.06 g）和干重（11.76 g），相对叶绿素含量（42.24）和Fv / Fm比（0.66）。。受混血双歧杆菌和色球菌影响的主要成分是柠檬烯，α- pine烯，γ-萜品烯，石竹烯氧化物，胚轴D和萜品烯-4-醇。精油成分的比例最高（97.6％）D. Kotschyi用20分钟，观察苗B. polymixa悬浮培养浸泡种子。结果表明，种子对B. polymixa的生物引发作用与D. Kotschyi的种子发芽，幼苗生长和精油产量相关的其他处理方法，持续20分钟比其他处理更有效。