当前位置: X-MOL 学术Xenobiotica › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
The biotransformation of Bupleuri Radix by human gut microbiota.
Xenobiotica ( IF 1.8 ) Pub Date : 2020-01-16 , DOI: 10.1080/00498254.2019.1707908
Cui Tang 1, 2, 3, 4 , Qiachi Fu 5 , Xia Chen 6 , Yang Hu 7 , Helen Renaud 8 , Chong Ma 1, 2, 3, 4 , Tai Rao 1, 2, 3, 4 , Yao Chen 1, 2, 3, 4 , Zhirong Tan 1, 2, 3, 4 , Curtis D Klaassen 8 , Shuyun Shi 5 , Ying Guo 1, 2, 3, 4
Affiliation  

1. Bupleuri Radix (BR) is a herbal medicine traditionally used orally in oriental countries, which inevitably comes into contact with the intestinal microbiota. However, whether gut microbiota contribute to the biotransformation of BR, and/or the formation of pharmacologically active compounds remains unknown.

2. In this study, the main saikosaponins (SAPs) of Bupleurum (including saikosaponin a, b1, b2, c, d, f, h) and BR extract (BRE) were individually incubated with human fecal suspensions (HFS), and metabolic time courses of SAPs and their metabolites by human gut bacteria were systematically characterized.

3. Deglycosylation and dehydration were the main metabolic pathways identified for SAPs including newly investigated saikosaponin f (SSf) and saikosaponin h (SSh); dehydration had not been reported previously. A total of 19 dehydrated and deglycosylated metabolites of SAPs were detected and characterized, and 10 of them were newly identified. Moreover, SAPs of BRE were found to be deglycosylated to prosaikogenins. In addition, 13 metabolic pathways related to human gut microbiota were identified for phytochemicals of BRE except for SAPs. Gut microbiota may play a significant role in the biotransformation of BR in humans.



中文翻译:

人肠道菌群对柴胡的生物转化。

1. Bupleuri Radix(BR)是东方国家传统上经口使用的草药,不可避免地会与肠道菌群接触。然而,肠道菌群是否有助于BR的生物转化和/或药理活性化合物的形成尚不清楚。

在这种研究中,主要的柴胡皂苷(SAPS)柴胡(包括柴胡皂苷A,B1,B2,C,d,F,H)和BR提取物(BRE)与人的粪便悬浮液(HFS)和代谢单独孵育系统分析了人类肠道细菌对SAP及其代谢产物的时程变化。

3.糖基化和脱水是SAPs鉴定的主要代谢途径,包括新研究的皂苷总皂苷f(SSf)和皂苷总皂苷h(SSh)。以前没有关于脱水的报道。总共检测和鉴定了19种脱水和去糖基化的SAP代谢物,其中10种是新鉴定的。此外,发现BRE的SAP被去糖基化为prosaikogenins。此外,除SAPs外,还发现了与BRE的植物化学物质有关的13种与人肠道菌群有关的代谢途径。肠道菌群可能在人类BR的生物转化中起重要作用。

更新日期:2020-01-16
down
wechat
bug