Genome size—the total haploid content of nuclear DNA— is constant in all cells in individuals within a species, but differs among species. Consequently, the genome size is a quantifiable genetic signature that not only characterizes a species, but it can reflect chromatin modifications, which play fundamental roles in most biological processes that are involved in the manipulation and expression of DNA. This characteristic makes the genome size a crucial parameter for genetic research on endemic aquatic species and for genetic manipulations in aquaculture species. Technologies for genetic assessments and improvements applied to fishery and aquaculture species use genome size values as a means by which hybrids, polyploids, and sex can be identified, when sex chromosomes exist. The objectives of this study were to determine genome sizes of aquatic species with economic and biological importance along the Pacific coast of Mexico, as well as to identify the appropriate reference standards for use in this study. Blood, hemolymph or milt were collected from 10 species occurring along the coast of Baja California: Sablefish Anoplopoma fimbria, Black Snapper Lutjanus novemfasciatus, California Halibut Paralichthys californicus, Pacific Sardine Sardinops sagax, Flag Rockfish Sebastes rubrivinctus, Starry Rockfish Sebastes constellatus, Totoaba Totoaba macdonaldi, Whiteleg Shrimp Litopenaeus vannamei and two Yellowtail Seriola lalandi and S. dorsalis. Nuclear DNA was stained with propidium iodide solution and the genome size was determined by flow cytometry, with results ranging from 0.61 pg (1.22 pg/diploid cell) to 2.59 pg (5.18 pg/diploid cell), with the smallest value in Sablefish and the largest in the Whiteleg Shrimp. No significant differences were detected (P ≤ 0.05) among individuals of the same species; the likely reason behind any dissimilar DNA content values with those from the literature were differences in methodologies or variations in genetics. Red-ear Slider Turtle Trachemys scripta elegans 2.65 pg (5.30 pg/diploid cell) and Red Junglefowl Gallus gallus 1.27 pg (2.54 pg/diploid cell) were chosen as the standards for reference values. These results establish the basis for the Mexican National Aquatic Genetic Resources project supporting genetic improvements for aquaculture and conservation status parameters for fisheries species.

基因组大小（核DNA的单倍体总含量）在一个物种内的所有细胞中都是恒定的，但在物种之间有所不同。因此，基因组大小是可量化的遗传特征，不仅可以表征一个物种，而且可以反映出染色质修饰，而染色质修饰在涉及DNA操纵和表达的大多数生物学过程中都起着基本作用。这一特征使基因组大小成为地方水生物种遗传研究和水产养殖物种遗传操作的关键参数。应用于渔业和水产养殖物种的遗传评估和改良技术利用基因组大小值作为当存在性染色体时可以鉴定杂种，多倍体和性别的手段。这项研究的目的是确定墨西哥太平洋沿岸具有经济和生物学重要性的水生物种的基因组大小，并确定用于本研究的适当参考标准。从下加利福尼亚州沿岸的10种物种中收集了血液，淋巴液或污垢：黑貂Anoplopoma伞，黑鲷笛novemfasciatus，加州大比目鱼牙加利福尼亚小新，太平洋沙丁鱼Sardinops sagax，旗石鱼平鲉属rubrivinctus，星夜罗克菲什平鲉属星座，加利福尼亚湾石首鱼加利福尼亚湾石首鱼macdonaldi，对虾凡纳滨对虾和两个黄尾鰤lalandi和S.背。核DNA用碘化丙啶溶液染色，并通过流式细胞仪确定基因组大小，结果范围从0.61 pg（1.22 pg /二倍体细胞）到2.59 pg（5.18 pg /二倍体细胞），在紫花鱼和白腿虾中最大的虾。未检测到显著差异（P  ≤0.05）相同物种的个体中; 与文献中的DNA含量值不同的背后原因可能是方法学差异或遗传学差异。红耳滑龟Trachemys elegans 2.65 pg（5.30 pg /二倍体细胞）和Red Junglefowl Gallus gallus选择1.27 pg（2.54 pg /二倍体细胞）作为参考值的标准。这些结果为墨西哥国家水生遗传资源项目提供了基础，该项目支持水产养殖的遗传改良和渔业物种的保护状况参数。