Arbuscular mycorrhizal fungi provide benefits to plants, especially under stressful growing conditions. These symbiotic fungi can be applied as biofertilizers prior to transplant in order to increase establishment success in the field. Roots are often trimmed at the time of transplant to reduce the probability of J-rooting, the upward orientation of roots within a planting hole which can lead to root death and disease. The effect of root trimming on the mycorrhizal symbiosis is unknown. It is possible that trimming may remove the active mycelium, nullifying the effect of inoculation. We conducted a greenhouse study to test the effect of root trimming on the mycorrhizal symbiosis in grapevine. The mycorrhizal symbiosis persisted after root trimming. Trimming reduced the abundance of AM fungi in older roots. The fungi were able to recolonize the new roots in trimmed vines, and these roots had more arbuscules compared to older roots, which had mostly vesicles. Trimmed vines had lower shoot, but not root, biomass. The mycorrhizal symbiosis persisted in the roots, despite trimming, likely due to fungal structures in older, untrimmed roots serving as propagules. We conclude that inoculation with AM fungi prior to field transplant is robust to root trimming, at least for the isolate examined in this study.

中文翻译：

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剪枝对葡萄砧木丛枝菌根共生的影响

丛枝菌根真菌为植物带来了好处，尤其是在胁迫性生长条件下。这些共生真菌可以在移植前用作生物肥料，以增加在现场的成功种植。在移植时经常修剪根部，以减少J植根的可能性，J植根是指种植孔中的根部朝上，这可能导致根系死亡和疾病。修剪根对菌根共生的影响尚不清楚。修剪可能会去除活性菌丝体，从而使接种效果无效。我们进行了温室研究，以测试修剪根部对葡萄菌根共生的影响。根部修剪后菌根共生持续。修剪减少了老根中AM真菌的含量。真菌能够使修剪过的葡萄藤中的新根重新定殖，与较老的根（多数为囊泡）相比，这些根具有更多的丛枝。修剪过的藤蔓的生物量较低，但没有根。尽管已修剪，但菌根共生在根部持续存在，这可能是由于较老的未修剪根部作为繁殖体的真菌结构所致。我们得出的结论是，至少在本研究中检测到的分离株中，在田间移植之前接种AM真菌对根部修剪很牢固。可能是由于较旧的未修剪根的真菌结构作为繁殖体。我们得出的结论是，至少在本研究中检测到的分离株中，在田间移植之前接种AM真菌对根部修剪很牢固。可能是由于较旧的未修剪根的真菌结构作为繁殖体。我们得出的结论是，至少在本研究中检测到的分离株中，在田间移植之前接种AM真菌对根部修剪很牢固。