A population of 206 recombinant inbred lines (RILs F₉–F₁₀) derived from wheat cross WL711/C306 was phenotyped for morpho-physiological traits such as flag leaf area (FLA), flag leaf length (FLL), flag leaf width (FLW), and cell membrane stability (CMS) under water deficit stress (WDS) environment. High yielding cultivar, WL711 had higher FLA than the medium yielding cultivar C306 across trials under both environments. Parent cultivar C306 maintained membrane integrity while WL711 showed higher membrane damage under WDS. The RIL population showed considerable variation, normal distribution and transgressive segregation for FLA, FLL, FLW and CMS under WDS. The genetic linkage map of WL711/C306 RIL population was constructed comprising of 346 markers. The total map distance was 4526.8 cM with an averaged interval of 12.9 cM between adjacent markers. Major consistent QTL for FLA, FLL, FLW, and CMS were identified on chromosomes 2DS and 3BS respectively in the WL711/C306 RIL population under WDS. The major QTL for FLA, qFLAWD.2D.1 which expressed in multiple environments and for CMS, qCMSWD.3B.3 and qCMSWD.3B.4, accounted for a large proportion of phenotypic variance (PV) with positive allele being contributed by C306, a drought resistant (DR) parent. QTL qFLAWD.2D.1 for FLA co-located with QTL for grain number (GN) and days to flowering (DTF) while QTL qCMSWD.3B.3 and qCMS.3B.4 co-located with QTL for grain yield and its components, days to flowering, canopy temperature and coleoptiles length as reported in our previous publications on the WL711/C306 population (Shukla et al. in Euphytica 203:449–467, 2015; Singh et al. in J Plant Biochem Biotechnol 24:324–330, 2015). Two candidate genes Ghd7 for grain yield and heading date and OsCDK4 for calcium dependent protein kinases were identified in the 2DS and 3BS QTL regions respectively on comparison with gene content of rice chromosomes 7 and 1 respectively. Hence, QTLs qFLAWD.2D.1 and qCMSWD.3B.3 are potential target regions for fine mapping and marker assisted selection for FLA and CMS respectively in wheat under water deficit environments.

中文翻译：

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小麦RIL群体干旱胁迫下细胞膜稳定性和旗叶面积的QTL

206个重组自交系（RIL F ₉ –F ₁₀对来自小麦杂交种WL711 / C306的小麦进行表型分析，以研究其水分亏缺胁迫下（旗叶面积（FLA），旗叶长度（FLL），旗叶宽度（FLW）和细胞膜稳定性（CMS））的形态生理特征。 WDS）环境。在两种环境下的所有试验中，WL711均具有高产量品种，而FL711高于中等产量品种C306。亲本品种C306保持膜完整性，而WL711在WDS下显示较高的膜损伤。在WDS下，RIL种群显示FLA，FLL，FLW和CMS的变异，正态分布和海侵分离。WL711 / C306 RIL群体的遗传连锁图谱由346个标记组成。总图谱距离为4526.8 cM，相邻标记之间的平均间隔为12.9 cM。FLA，FLL，FLW的主要一致QTL，WDS下WL711 / C306 RIL群体的2DS和3BS染色体上分别鉴定到了CMS和CMS。FLA的主要QTL，qFLAWD.2D.1这在多种环境和CMS，表示qCMSWD.3B.3和qCMSWD.3B.4，占了很大的比例表型变异（PV）与阳性等位基因被贡献的C306，一个抗旱（DR ）父母。用于FLA的QTL qFLAWD.2D.1与用于谷物数量（GN）和开花天数（DTF）的QTL位于同一位置，而用于谷物产量及其组成部分的QTL qCMSWD.3B.3和qCMS.3B.4与QTL位于同一位置，开花天数，冠层温度和胚芽鞘长度，如我们先前关于WL711 / C306种群的出版物所述（Shukla等人在Euphytica 203：449-467，2015； Singh等人在J Plant Biochem Biotechnol 24：324- 330，2015年）。两个候选基因Ghd7通过与水稻第7号和第1号染色体的基因含量比较，分别在2DS和3BS QTL区域确定了谷物产量和抽穗期的OsCDK4和钙依赖性蛋白激酶的OsCDK4。因此，QTL qFLAWD.2D.1和qCMSWD.3B.3是潜在缺水环境下小麦中FLA和CMS的精细作图和标记辅助选择的潜在目标区域。