BackgroundLisinopril (LNP) is an angiotensin-converting enzyme inhibitor used as anti-hypertensive, cardiovascular, in anti-prophylactic and anti-diabetic nephropathy drug. Development of two new, simple, low cost, and selective membrane-based ion-selective electrodes has been proposed for the determination of LNP in pharmaceuticals.MethodsThe electrodes are based on poly(vinyl)chloride membrane doped with LNP-phosphotungstic acid (LNP-PTA) and LNP-phosphomolybdic acid (LNP-PMA) ion-pairs as molecular recognition materials.ResultsThe developed LNP-PTA and LNP-PMA electrodes are applicable for the determination of LNP over the linear range of 5 × 10−5–2.4 × 10−3 mol l−1. The working pH ranges to measure potentials were 2.5 to 6.4 and 2.3 to 6.0 for LNP-PTA and LNP-PMA ISEs, respectively. The electrodes displayed the rapid Nernstian responses as revealed by the values of slopes 55.06 and 52.39 mV/decade, with limit of detection (LOD) values of 1.2 × 10−5 and 1.18 × 10−5 mol l−1 for LNP-PTA and LNP-PMA electrodes, respectively. The limits of quantitation (LOQ) values have also been calculated for both the electrodes. The developed electrodes have potential stability for up to 1 month and emerged as highly selective for the determination of LNP over other spiked ions and compounds.ConclusionsThe proposed electrodes have been validated and found that they are suitable for the determination of LNP in pharmaceuticals in pure form and in dosage forms. The results obtained in the analysis of LNP using proposed electrodes have been compared statistically with reference method’s results to assess the accuracy and precision. Robustness and ruggedness of the developed electrodes have also been checked and found satisfactory. The recovery studies have been performed by standard addition procedure to assess the role of excipients in tablets containing LNP and the results obtained are satisfactory.

中文翻译：

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用于选择性测定药物中赖诺普利的膜电极的研制

背景赖诺普利 (LNP) 是一种血管紧张素转换酶抑制剂，用作抗高血压、心血管、抗预防和抗糖尿病肾病药物。已提议开发两种新型、简单、低成本、选择性的膜基离子选择性电极，用于测定药物中的 LNP。方法电极基于掺杂有 LNP-磷钨酸 (LNP- PTA) 和 LNP-磷钼酸 (LNP-PMA) 离子对作为分子识别材料。 结果开发的 LNP-PTA 和 LNP-PMA 电极适用于在 5 × 10−5–2.4 × 线性范围内测定 LNP 10-3 mol l-1。对于 LNP-PTA 和 LNP-PMA ISE，测量电位的工作 pH 范围分别为 2.5 至 6.4 和 2.3 至 6.0。电极显示出快速的 Nernstian 响应，如斜率 55.06 和 52.39 mV/decade 所示，LNP-PTA 的检测限 (LOD) 值为 1.2 × 10-5 和 1.18 × 10-5 mol l-1 和分别为 LNP-PMA 电极。还计算了两个电极的定量限 (LOQ) 值。开发的电极具有长达 1 个月的潜在稳定性，并且比其他加标离子和化合物对 LNP 的测定具有高度的选择性。结论所提出的电极已经过验证，发现它们适用于测定纯药物中的 LNP和剂型。使用建议的电极分析 LNP 中获得的结果已与参考方法的结果进行统计比较，以评估准确度和精密度。开发的电极的坚固性和坚固性也已经过检查并发现令人满意。回收率研究是通过标准添加程序进行的，以评估赋形剂在含有 LNP 的片剂中的作用，获得的结果令人满意。