Viruses have evolved many mechanisms to invade host cells and establish successful infections. The interaction between viral attachment proteins and host cell receptors is the first and decisive step in establishing such infections, initiating virus entry into the host cells. Therefore, the identification of host receptors is fundamental in understanding pathogenesis and tissue tropism. Furthermore, receptor identification can inform the development of antivirals, vaccines, and diagnostic technologies, which have a substantial impact on human health. Nevertheless, due to the complex nature of virus entry, the redundancy in receptor usage, and the limitations in current identification methods, many host receptors remain elusive. Recent advances in targeted gene perturbation, high-throughput screening, and mass spectrometry have facilitated the discovery of virus receptors in recent years. In this review, we compare the current methods used within the field to identify virus receptors, focussing on genomic- and interactome-based approaches.

中文翻译：

---

鉴定病毒受体的高通量方法的进展。

病毒已经进化出许多入侵宿主细胞并建立成功感染的机制。病毒附着蛋白与宿主细胞受体之间的相互作用是建立这种感染并启动病毒进入宿主细胞的决定性的第一步。因此，鉴定宿主受体是理解发病机理和组织嗜性的基础。此外，受体识别可以为抗病毒药，疫苗和诊断技术的发展提供信息，这对人类健康具有重大影响。然而，由于病毒进入的复杂性，受体使用的冗余以及当前鉴定方法的局限性，许多宿主受体仍然难以捉摸。靶向基因干扰，高通量筛选，近年来，质谱和质谱法促进了病毒受体的发现。在这篇综述中，我们比较了目前在该领域内用于鉴定病毒受体的方法，重点是基于基因组和相互作用组的方法。