Abstract

The review summarizes the problems, achievements, and prospects of various approaches related to the delivery of components of DNA editing systems to plant cells, the regeneration of whole plants with an edited genome, and the development of transgene-free or transgene-clean crops. Here, an attempt was made to systematize the results of various studies describing the successful production of genome-edited (GE) plants with various genome modifications/mutations via the application of nuclease DNA–editing systems (ZFN, TALEN, and CRISPR/Cas). We discuss the main directions for the development of nuclease-based genome-editing methods to obtain GE plants free of foreign sequences from genome-editing tools.

中文翻译：

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非转基因，基因组编辑植物生产的成就，挑战和前景

摘要

这篇综述总结了与将DNA编辑系统的成分传递给植物细胞，基因组经过编辑的整株植物的再生以及无转基因或无转基因作物的发展有关的各种方法的问题，成就和前景。在这里，我们试图将各种研究的结果系统化，这些研究描述了通过应用核酸酶DNA编辑系统（ZFN，TALEN和CRISPR / Cas）成功生产具有各种基因组修饰/突变的基因组编辑（GE）植物。我们讨论了开发基于核酸酶的基因组编辑方法的主要方向，以从基因组编辑工具中获取不含外源序列的GE植物。