Lineage specification in early development is the basis for the exquisitely precise body plan of multicellular organisms. It is therefore critical to understand cell fate decisions in early development. Moreover, for regenerative medicine, the accurate specification of cell types to replace damaged/diseased tissue is strongly dependent on identifying determinants of cell identity. Long noncoding RNAs (lncRNAs) have been shown to regulate cellular plasticity, including pluripotency establishment and maintenance, differentiation and development, yet broad phenotypic analysis and the mechanistic basis of their function remains lacking. As components of molecular condensates, lncRNAs interact with almost all classes of cellular biomolecules, including proteins, DNA, mRNAs, and microRNAs. With functions ranging from controlling alternative splicing of mRNAs, to providing scaffolding upon which chromatin modifiers are assembled, it is clear that at least a subset of lncRNAs are far from the transcriptional noise they were once deemed. This review highlights the diversity of lncRNA interactions in the context of cell fate specification, and provides examples of each type of interaction in relevant developmental contexts. Also highlighted are experimental and computational approaches to study lncRNAs.

中文翻译：

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多模式长非编码 RNA 相互作用网络：细胞命运规范的控制面板。

早期发育中的谱系规范是多细胞生物精确的身体规划的基础。因此，了解早期发育中的细胞命运决定至关重要。此外，对于再生医学来说，替代受损/患病组织的细胞类型的准确规范在很大程度上取决于细胞身份决定因素的识别。长非编码 RNA (lncRNA) 已被证明可以调节细胞可塑性，包括多能性的建立和维持、分化和发育，但仍缺乏广泛的表型分析及其功能的机制基础。作为分子凝聚物的组成部分，lncRNA 与几乎所有类别的细胞生物分子相互作用，包括蛋白质、DNA、mRNA 和 microRNA。其功能范围从控制 mRNA 的选择性剪接，到提供组装染色质修饰剂的支架，很明显，至少有一部分 lncRNA 与它们曾经被认为的转录噪音相去甚远。这篇综述强调了细胞命运规范背景下 lncRNA 相互作用的多样性，并提供了相关发育背景下每种类型相互作用的示例。还强调了研究 lncRNA 的实验和计算方法。