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Low prevalence of human papillomavirus infection in sinonasal inverted papilloma and oncocytic papilloma.
Virchows Archiv ( IF 3.5 ) Pub Date : 2019-12-03 , DOI: 10.1007/s00428-019-02717-3
Huan Wang 1 , Changwen Zhai 2 , Juan Liu 1 , Jingjing Wang 1 , Xicai Sun 1 , Li Hu 1, 3, 4 , Dehui Wang 1
Affiliation  

The aim of this study was to investigate the role of human papillomavirus (HPV) in sinonasal inverted papilloma (SIP) and sinonasal oncocytic papilloma (SOP) from a single institution and whether p16 can serve as a surrogate marker for HPV infection. This study included 49 subjects with SIP and 36 subjects with SOP. Formalin-fixed paraffin-embedded tissues were used to extract genomic DNA, and HPV detection was performed by utilizing a valid nested polymerase chain reaction approach that can detect all known HPV subtypes. Immunohistochemistry was used to evaluate the expression of p16 in all tumor sections. The presence of HPV DNA was found in 6.1% (3/49) of the SIP patients and 11.1% (4/36) of the SOP patients. All identified HPV subtypes in SIP were high-risk HPV, including HPV-16 (two patients) and HPV-58 (one patient). Regarding SOP, there were three patients positive for HPV-16 and one with low-risk HPV (type 6). In total, 11/49 (22.4%) SIP lesions and 10/36 (27.8%) SOP lesions were considered p16 positive, with p16 staining in more than 70% of tumor cells. There was only one SIP and one SOP that were positive for both HPV (high-risk HPV type 16) and p16 staining. HPV does not play an etiologic role in inverted papilloma or oncocytic papilloma of the sinonasal region. p16 immunostaining should not be used as a surrogate marker to evaluate the HPV infection status in these lesions.

中文翻译:

人乳头瘤病毒感染在鼻鼻窦倒置性乳头瘤和囊细胞性乳头瘤中的感染率较低。

这项研究的目的是调查人类乳头瘤病毒(HPV)在单个机构的鼻窦倒置性乳头状瘤(SIP)和鼻腔鼻囊性乳头状瘤(SOP)中的作用,以及p16是否可以作为HPV感染的替代标志物。这项研究包括49名SIP患者和36名SOP患者。用福尔马林固定的石蜡包埋的组织提取基因组DNA,并利用有效的巢式聚合酶链反应方法进行HPV检测,该方法可以检测所有已知的HPV亚型。免疫组织化学用于评估所有肿瘤切片中p16的表达。在6.1%(3/49)的SIP患者和11.1%(4/36)的SOP患者中发现HPV DNA的存在。SIP中所有已鉴定的HPV亚型均为高危HPV,包括HPV-16(两名患者)和HPV-58(一名患者)。关于SOP,有3例HPV-16阳性和1例低危HPV(6型)。总共有11/49(22.4%)SIP病变和10/36(27.8%)SOP病变被认为是p16阳性,超过70%的肿瘤细胞被p16染色。HPV(高危型HPV 16型)和p16染色均为阳性的SIP和1个SOP。HPV在鼻窦区域的倒置乳头状瘤或囊性乳头状瘤中没有病因学作用。p16免疫染色不应用作评估这些病变中HPV感染状况的替代指标。HPV(高危型HPV 16型)和p16染色均为阳性的SIP和1个SOP。HPV在鼻窦区域的倒置乳头状瘤或囊性乳头状瘤中没有病因学作用。p16免疫染色不应用作评估这些病变中HPV感染状况的替代指标。HPV(高危型HPV 16型)和p16染色均为阳性的SIP和1个SOP。HPV在鼻窦区域的倒置乳头状瘤或囊性乳头状瘤中没有病因学作用。p16免疫染色不应用作评估这些病变中HPV感染状况的替代指标。
更新日期:2020-04-18
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