Justicia secunda Vahl. is a medicinal plant used in ethnomedical practice as therapy to manage inflammation. Therefore, this study was designed to evaluate the anti-inflammatory activity of methanol extract of J. secunda leaves (MEJSL) using in vitro and in vivo inflammation models. Seventy-percent MEJSL was prepared following standard procedure. In vitro anti-inflammatory assays were performed using heat-induced bovine serum albumin (BSA) denaturation and erythrocyte membrane stabilization assays. Carrageenan and formaldehyde induced inflammation in rat models were used to evaluate the anti-inflammatory activity of MEJSL in vivo. Diclofenac sodium was used as a reference drug. In addition, liver and kidney function assays and hematological analysis were carried out. Data revealed that varying concentrations of MEJSL significantly (P < 0.05) inhibited heat-induced BSA denaturation and stabilized erythrocyte membrane against hypotonicity-induced hemolysis when compared with diclofenac sodium in a concentration-dependent manner. In vivo study showed that 10 mg/kg body weight (b.w.) diclofenac sodium, 100 and 300 mg/kg b.w. MEJSL suppressed carrageenan-induced paw edema at the sixth hour by 71.14%, 83.08%, and 89.05%, respectively. Furthermore, 10 mg/kg b.w. diclofenac sodium, 100 and 300 mg/kg b.w. MEJSL inhibited formaldehyde-induced paw edema by 72.53%, 74.73%, and 76.48%, respectively. Animals treated with varying doses of MEJSL had reduced plasma aspartate aminotransferase and alanine aminotransferase activities; urea and creatinine concentrations; and modulated hematological parameters when compared with the untreated control group. Findings from this study showed that MEJSL exhibited substantial anti-inflammatory actions in the in vitro and in vivo models. It also indicated that MEJSL anti-inflammatory mechanisms of action could be through interference with phase 2 inflammatory stressors, upregulation of cytoprotective genes, stabilization of inflammatory cell membranes and immunomodulatory activity.

中文翻译：

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的抗炎活性的评价正义塞康达使用体外和体内炎症模型干旱胁迫下连翘叶提取物

Justicia secunda Vahl。是一种在民族实践中用作治疗炎症的药物。因此，本研究旨在使用体外和体内炎症模型评估苏枯草叶甲醇提取物（MEJSL）的抗炎活性。按照标准程序制备百分之七十的MEJSL。使用热诱导的牛血清白蛋白（BSA）变性和红细胞膜稳定化测定进行体外抗炎测定。角叉菜胶和甲醛诱发的大鼠炎症用于评估MEJSL的体内抗炎活性。双氯芬酸钠被用作参考药物。此外，还进行了肝肾功能测定和血液学分析。数据显示，MEJSL的浓度变化显着（P <0。05）与双氯芬酸钠相比，以浓度依赖性方式抑制热诱导的BSA变性并稳定红细胞膜以抵抗低渗性引起的溶血。体内研究表明，在第六个小时，体重10毫克/千克体重（bw）的双氯芬酸钠，100毫克/千克体重和300毫克/千克体重的MEJSL抑制了角叉菜胶诱发的爪水肿，分别为71.14％，83.08％和89.05％。此外，10 mg / kg bw双氯芬酸钠，100 mg / kg bw和300 mg / kg bw MEJSL分别抑制甲醛引起的爪水肿，分别为72.53％，74.73％和76.48％。用不同剂量的MEJSL治疗的动物血浆天冬氨酸转氨酶和丙氨酸转氨酶活性降低；尿素和肌酐浓度；与未经治疗的对照组相比，血液学参数得到了调节。这项研究的发现表明，MEJSL在体外和体内模型中均表现出明显的抗炎作用。它还表明，MEJSL的抗炎作用机制可能是通过干扰2期炎症应激源，上调细胞保护基因，稳定炎症细胞膜和免疫调节活性。