当前位置：
X-MOL 学术
›
Plant Sci.
›
论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Gibberellin recovers seed germination in rice with impaired brassinosteroid signalling
Plant Science ( IF 5.2 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.plantsci.2020.110435 Qian-Feng Li 1 , Yu Zhou 2 , Min Xiong 2 , Xin-Yu Ren 2 , Li Han 2 , Jin-Dong Wang 2 , Chang-Quan Zhang 1 , Xiao-Lei Fan 1 , Qiao-Quan Liu 1
Plant Science ( IF 5.2 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.plantsci.2020.110435 Qian-Feng Li 1 , Yu Zhou 2 , Min Xiong 2 , Xin-Yu Ren 2 , Li Han 2 , Jin-Dong Wang 2 , Chang-Quan Zhang 1 , Xiao-Lei Fan 1 , Qiao-Quan Liu 1
Affiliation
Seed germination is essential for ensuring grain yield and quality. Germination rate, uniformity, and post-germination growth all contribute to cultivation. Although the phytohormones gibberellin (GA) and brassinosteroid (BR) are known to regulate germination, the underlying mechanism of their crosstalk in co-regulating rice seed germination remains unclear. In this study, the isobaric tags for relative and absolute quantitation (iTRAQ) proteomic approach was employed to identify target proteins responsive to GA during recovery of germination in BR-deficient and BR-insensitive rice. A total of 42 differentially abundant proteins were identified in both BR-deficient and BR-insensitive plants, and most were altered consistently in the two groups. Gene Ontology (GO) analysis revealed enrichment in proteins with binding and catalytic activity. A potential protein-protein interaction network was constructed using STRING analysis, and five Late Embryogenesis Abundant (LEA) family members were markedly down-regulated at both mRNA transcript and protein levels. These LEA genes were specifically expressed in rice seeds, especially during the latter stages of seed development. Mutation of LEA33 affected rice grain size and seed germination, possibly by reducing BR accumulation and enhancing GA biosynthesis. The findings improve our knowledge of the mechanisms by which GA and BR coordinate seed germination.
更新日期：2020-04-01
京公网安备 11010802027423号