Flow cytometric basophil activation tests: Staining of exteriorized basophil granule matrix by fluorescent avidin versus appearance of CD63.,Cytometry Part B: Clinical Cytometry

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Flow cytometric basophil activation tests: Staining of exteriorized basophil granule matrix by fluorescent avidin versus appearance of CD63.
Cytometry Part B: Clinical Cytometry ( IF 3.4 ) Pub Date : 2020-02-03 , DOI: 10.1002/cyto.b.21868
Didier G Ebo _{1,

2} , Jessy Elst ₁ , Michel van Houdt ₁ , Isabel Pintelon ₃ , Jean-Pierre Timmermans ₃ , Tatsuo Horiuchi ₄ , Margaretha A Faber ₁ , Margo M Hagendorens _{1,

5} , Christel M Mertens ₁ , Vito Sabato _{1,

2}

Affiliation

Staining of exteriorized basophil granule matrix by fluorescent avidin might be a reliable technique to monitor basophil degranulation. This study compares the avidin‐based technique with the upregulation of CD203c and appearance of CD63 in response to various stimuli.

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