BACKGROUND Cyclosporine-A has been regarded as an immunoregulatory and anti-inflammatory drug for the treatment of various immune inflammatory diseases. However, the effect of Cyclosporine-A on the retina of type 2 diabetic rats and the underlying mechanism remains to be elucidated. The objective of the present study was to investigate the effect and mechanism of Cyclosporine-A on diabetic retinopathy. METHODS Male Sprague-Dawley rats were established to type 2 diabetic model. After 6 weeks, diabetic rats and normal controls were intravitreally injected with. Cs-A (42 ng/2 μL) to the left eye, and 2 μL DMSO to the right eye for the control.. Another group of normal wild-type rats was subjected to intravitreal injections into. The left eyes with 5 μL PBS or HMGB-1 (5 ng/5 μL) or HMGB-1(5 ng/5 μL) plus. Cs-A (42 ng/2 μL), respectively. Retinal morphological changes were observed with. Hematoxylin-eosin staining. Expressions of HMGB-1, IL-1β and TNF-α were. Detected by immunohistochemistry, ELISA or Western blot or RT-PCR. RESULTS Retinal expression levels of IL-1β and TNF-α were upregulated in type 2. diabetic rats and in normal rats with intravitreal injection of HMGB-1, which were. Attenuated by intravitreal Cs-A. Moreover, Cs-A decreased HMGB-1 expression in. diabetic retina and relieved the retinopathy in type 2 diabetic rats. CONCLUSIONS Intravitreal administration of Cs-A showed a protective effect on retina. of diabetic rats, possibly by downregulating retinal expressions of IL-1β and TNF-α. via the suppression of HMGB-1.

中文翻译：

---

环孢菌素-a通过抑制2型糖尿病大鼠的HMGB-1形成来减轻视网膜炎症。

背景技术环孢菌素-A已经被认为是用于治疗各种免疫炎性疾病的免疫调节和抗炎药。然而，环孢菌素A对2型糖尿病大鼠视网膜的作用及其潜在机制仍有待阐明。本研究的目的是研究环孢菌素A对糖尿病性视网膜病变的作用和机制。方法建立雄性Sprague-Dawley大鼠为2型糖尿病模型。6周后，玻璃体内注射糖尿病大鼠和正常对照组。左眼为Cs-A（42 ng / 2μL），右眼为2μLDMSO。对另一组正常野生型大鼠进行玻璃体内注射。左眼用5μLPBS或HMGB-1（5 ng / 5μL）或HMGB-1（5 ng / 5μL）加。Cs-A（42 ng / 2μL）。观察到视网膜形态变化。苏木精-伊红染色。HMGB-1，IL-1β和TNF-α的表达分别为。通过免疫组织化学，ELISA或Western blot或RT-PCR检测。结果在2型糖尿病大鼠和玻璃体腔注射HMGB-1的正常大鼠中，IL-1β和TNF-α的视网膜表达水平上调。玻璃体内Cs-A减弱。此外，Cs-A降低了2型糖尿病大鼠糖尿病视网膜中HMGB-1的表达，减轻了视网膜病变。结论玻璃体内给予Cs-A对视网膜具有保护作用。可能通过下调IL-1β和TNF-α的视网膜表达来控制糖尿病大鼠的行为。通过抑制HMGB-1。ELISA或Western印迹或RT-PCR。结果在2型糖尿病大鼠和玻璃体腔注射HMGB-1的正常大鼠中，IL-1β和TNF-α的视网膜表达水平上调。玻璃体内Cs-A减弱。此外，Cs-A降低了2型糖尿病大鼠糖尿病视网膜中HMGB-1的表达，减轻了视网膜病变。结论玻璃体内给予Cs-A对视网膜具有保护作用。可能通过下调IL-1β和TNF-α的视网膜表达来控制糖尿病大鼠的行为。通过抑制HMGB-1。ELISA或Western印迹或RT-PCR。结果在2型糖尿病大鼠和玻璃体腔注射HMGB-1的正常大鼠中，IL-1β和TNF-α的视网膜表达水平上调。玻璃体内Cs-A减弱。此外，Cs-A降低了2型糖尿病大鼠糖尿病视网膜中HMGB-1的表达，减轻了视网膜病变。结论玻璃体内给予Cs-A对视网膜具有保护作用。可能通过下调IL-1β和TNF-α的视网膜表达来控制糖尿病大鼠的行为。通过抑制HMGB-1。糖尿病视网膜和减轻2型糖尿病大鼠的视网膜病变。结论玻璃体内给予Cs-A对视网膜具有保护作用。可能通过下调IL-1β和TNF-α的视网膜表达来控制糖尿病大鼠的行为。通过抑制HMGB-1。糖尿病视网膜和减轻2型糖尿病大鼠的视网膜病变。结论玻璃体内给予Cs-A对视网膜具有保护作用。可能通过下调IL-1β和TNF-α的视网膜表达来控制糖尿病大鼠的行为。通过抑制HMGB-1。