Herein, we introduce a protocol for preparing liposomes using high-pressure CO₂, water and direct ultrasonication (HPC-D) allowing rapid formation of liposomes in a single-step. In the HPC-D method, phospholipid suspensions were treated at temperatures from 25 °C to 70 °C and pressures from 4 MPa to 6.8 MPa with direct ultrasonication. The liposomes produced from HPC-D had an average particle size of 159 ± 2 nm to 136 ± 8 nm at liposome recovery yields up to 95.3 ± 4.6 %. A mechanism for the HPC-D method is proposed, in which the micro-phase separation between water-CO₂ interface increases surface area and phospholipids act as surfactants and reassemble into small liposome particles. Drug loading (DL) and encapsulation efficiency (EE) of liposomes obtained with the HPC-D method for Cyclosporin A gave DL values of 37.4 ± 3.4 % and EE of 79.7 ± 2.5 %, confirming efficient entrapment.

在本文中，我们介绍了一种使用高压CO ₂，水和直接超声（HPC-D）制备脂质体的方案，该步骤可在一个步骤中快速形成脂质体。在HPC-D方法中，直接超声处理在25°C至70°C的温度和4 MPa至6.8 MPa的压力下处理磷脂悬浮液。由HPC-D生产的脂质体的平均粒径为159±2 nm至136±8 nm，脂质体的回收率高达95.3±4.6％。提出了一种HPC-D方法的机理，其中水-CO ₂之间的微相分离界面增加表面积，磷脂充当表面活性剂并重新组装成小的脂质体颗粒。用HPC-D方法测得的环孢菌素A的脂质体的载药量（DL）和包封效率（EE）得到的DL值为37.4±3.4％，EE为79.7±2.5％，证实了有效的包封。