Extracellular vesicles (EVs) are small, heterogeneous and difficult to measure. Flow cytometry (FC) is a key technology for the measurement of individual particles, but its application to the analysis of EVs and other submicron particles has presented many challenges and has produced a number of controversial results, in part due to limitations of instrument detection, lack of robust methods and ambiguities in how data should be interpreted. These complications are exacerbated by the field's lack of a robust reporting framework, and many EV-FC manuscripts include incomplete descriptions of methods and results, contain artefacts stemming from an insufficient instrument sensitivity and inappropriate experimental design and lack appropriate calibration and standardization. To address these issues, a working group (WG) of EV-FC researchers from ISEV, ISAC and ISTH, worked together as an EV-FC WG and developed a consensus framework for the minimum information that should be provided regarding EV-FC. This framework incorporates the existing Minimum Information for Studies of EVs (MISEV) guidelines and Minimum Information about a FC experiment (MIFlowCyt) standard in an EV-FC-specific reporting framework (MIFlowCyt-EV) that supports reporting of critical information related to sample staining, EV detection and measurement and experimental design in manuscripts that report EV-FC data. MIFlowCyt-EV provides a structure for sharing EV-FC results, but it does not prescribe specific protocols, as there will continue to be rapid evolution of instruments and methods for the foreseeable future. MIFlowCyt-EV accommodates this evolution, while providing information needed to evaluate and compare different approaches. Because MIFlowCyt-EV will ensure consistency in the manner of reporting of EV-FC studies, over time we expect that adoption of MIFlowCyt-EV as a standard for reporting EV- FC studies will improve the ability to quantitatively compare results from different laboratories and to support the development of new instruments and assays for improved measurement of EVs.

中文翻译：

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MIFlowCyt-EV：用于细胞外囊泡流式细胞术实验的标准化报告的框架。

细胞外囊泡（EVs）小，异质且难以测量。流式细胞仪（FC）是测量单个颗粒的关键技术，但是将其应用于EV和其他亚微米颗粒的分析面临许多挑战，并产生了许多有争议的结果，部分原因是仪器检测的局限性，在如何解释数据方面缺乏可靠的方法和模棱两可。由于缺乏可靠的报告框架，这些并发症变得更加严重，许多EV-FC手稿包括对方法和结果的不完整描述，包含因仪器灵敏度不足和实验设计不当以及缺乏适当的校准和标准化而产生的假象。为了解决这些问题，一个由ISEV，ISAC和ISTH的EV-FC研究人员组成的工作组（WG）作为EV-FC工作组共同工作，并针对应提供的有关EV-FC的最低信息制定了共识框架。该框架将特定的EV-FC报告框架（MIFlowCyt-EV）纳入了现有的EV研究最低信息（MISEV）指南和FC实验（MIFlowCyt）标准的最低信息，该框架支持报告与样品染色相关的关键信息，EV检测和测量以及报告EV-FC数据的手稿中的实验设计。MIFlowCyt-EV提供了一种共享EV-FC结果的结构，但是它没有规定特定的协议，因为在可预见的将来，仪器和方法将继续快速发展。MIFlowCyt-EV适应了这一发展，同时提供评估和比较不同方法所需的信息。由于MIFlowCyt-EV将确保报告EV-FC研究的方式保持一致，因此随着时间的流逝，我们期望采用MIFlowCyt-EV作为报告EV-FC研究的标准将提高定量比较来自不同实验室的结果以及支持开发新的仪器和检测方法，以改善电动汽车的测量。